Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-220

Description :

The protein contains the fragments of the NS4 immunodominant region. Hepatitis C Virus NS4 proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HCV NS4 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25mM Tris-HCl, 1mM EDTA, 1,5M urea and 50% glycerol.

Storage:

Recombinant HCV NS4 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HCV NS4 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals.

Application:

Recombinant HCV NS4 Antigen may be used in ELISA and Western blots, excellent for detection of HCV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.
J Virol 2005 Oct;79(19):12425-33
2. Low-level hepatitis C viremia and humoral immune response to NS4 in chronic hepatitis B virus-hepatitis C virus coinfection.
Scand J Gastroenterol 2004 Aug;39(8):778-82
3. [Detection of type specific antibodies to hepatitis C virus NS4 protein in hepatitis C patients by enzyme immunoassay]
Vopr Virusol 2003 May-Jun;48(3):32-6
4. [Detection of hepatitis C virus-C, E, NS3 and NS4 regions' antigens in hepatocellular carcinoma and liver cirrhosis]
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 1998 Dec;12(4):326-9
5. Characterization of monoclonal antibodies and epitope mapping of the NS4 protein of hepatitis C virus.
Immunol Lett 2002 Oct 1;83(3):187-96
6. [Gene chimeric fusion and expression of nucleocapsid NS3 regions and NS4 regions of hepatitis C virus genome]
Sheng Wu Gong Cheng Xue Bao 2001 Jan;17(1):46-9