Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-256

Background:

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood.Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways
In addition,HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

Description :

The protein contains the HCV nonstructural NS3 protein immunodominant regions. Hepatitis C Virus NS3 proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HCV NS3 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/155痞 in 25mM Tris-Hcl pH 8, 1mM EDTA, 1.5M urea and 50% glycerol.

Storage:

Recombinant HCV NS3 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HCV NS3 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals

Application:

Recombinant HCV NS3 Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP.
Nature 2006 Jan 5 ;439(7072):105-8
2. [Preparation of recombinant IL-1 fusion protein as the substrate for HCV NS3 serine protease.]
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2006 Jan;22(1):43-6
3. Depeptidization efforts on P(3)-P(2)(') alpha-ketoamide inhibitors of HCV NS3-4A serine protease: Effect on HCV replicon activity.
Bioorg Med Chem Lett 2005 Dec 29;
4. Target HCV NS3 CD4(+) Th1 Epitope to Major Histocompatibility Complex Class II Pathway.
Biotechnol Lett 2006 Jan;28(1):3-8
5. [A study on the inhibitory effect on transplanted tumor growth expressing HCV NS3 protein by HCV minigene vaccine based on invariant chain substitution in mice]
Zhonghua Gan Zang Bing Za Zhi 2005 Nov;13(11):801-4
6. Discovery of small-molecule inhibitors of HCV NS3-4A protease as potential therapeutic agents against HCV infection.
Curr Med Chem 2005;12(20):2317-42