Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-244

Background:

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood.Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways
In addition,HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

Description :

The protein contains the HCV non structural protein NS3 immunodominant regions. Hepatitis C Virus NS3 proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HCV core 3 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25mM Tris-Hcl pH 8, 1mM EDTA, 1.5M urea and 50% glycerol.

Storage:

Recombinant HCV NS3 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HCV NS3 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals

Application:

Recombinant HCV NS3 Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP.
Nature 2006 Jan 5; 439(7072):105-8
2. Discovery of small-molecule inhibitors of HCV NS3-4A protease as potential therapeutic agents against HCV infection.
Curr Med Chem 2005;12(20):2317-42
3. Synthesis and biological activity of macrocyclic inhibitors of hepatitis C virus (HCV) NS3 protease.
Bioorg Med Chem Lett 2005 Oct 15;15(20):4475-8
4. Investigation of glycine alpha-ketoamide HCV NS3 protease inhibitors: effect of carboxylic acid isosteres.
Bioorg Med Chem Lett 2005 Aug 1;15(15):3487-90
5. Interaction between the HCV NS3 protein and the host TBK1 protein leads to inhibition of cellular antiviral responses.
Hepatology 2005 May;41(5):1004-12
6. Rational design of dual-functional aptamers that inhibit the protease and helicase activities of HCV NS3.
J Biochem (Tokyo) 2005 Mar;137(3):339-47