Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-266

Description:

E.coli derived recombinant from HCV subtype 5.
The protein contains the full-length HCV NS3 immunodominant region.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Formulation:

1mg/ml in 25mM Tris-HCl, 1mM EDTA, 1,5M urea and 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20₀C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals, including sera with antibodies to NS3 secondary domains.

Application:

Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP.
Nature 2006 Jan 5;43 9(7072):105-8
2. Discovery of small-molecule inhibitors of HCV NS3-4A protease as potential therapeutic agents against HCV infection.
Curr Med Chem 2005;12(20):2317-42
3. Synthesis and biological activity of macrocyclic inhibitors of hepatitis C virus (HCV) NS3 protease.
Bioorg Med Chem Lett 2005 Oct 15;15(20):4475-8
4. Investigation of glycine alpha-ketoamide HCV NS3 protease inhibitors: effect of carboxylic acid isosteres.
Bioorg Med Chem Lett 2005 Aug 1;15(15):3487-90
5. In teraction between the HCV NS3 protein and the host TBK1 protein leads to inhibition of cellular antiviral responses.
Hepatology 2005 May;41(5):1004-12
6. Rational design of dual-functional aptamers that inhibit the protease and helicase activities of HCV NS3.
J Biochem (Tokyo) 2005 Mar;137(3):339-47