Certificate of Analysis and Data Sheet

Source:
Pichia pastoris

Catalog No.
HBS-880

Physical appearance:

Sterile Filtered colourless liquid formulation

Formulation:

0.1M phosphate buffer pH 7.2-7.4, 0.15M sodium chloride, and 0.05 % sodium azide as preservative.

Stability:

Recombinant Hepatitis B Surface Antigen should be stored at 4蚓 between 3-4 weeks and for future use should be stored desiccated below -18蚓 .

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie staining gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

Was tested in ELISA with anti-HBsAg antibodies

Applications:

1. Immunochromatography (capture and conjugate).
2. Immunogen for monoclonal antibody production.
3. ELISA

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Dimers and aggregates:

Less than 0.1 ng/ug (IEU/ug) of HBsAg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Sequence analysis of hepatitis B virus genome of a new mutant of ayw subtype isolated in Sardinia.
Nucleic Acids Res 1991 Sep 25;19(18):5078
2. DNA cloned from the ayw subtype of hepatitis B virus.
J Med Virol 1980;6(2):139-45