Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HAV-225

Background:

HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.

Description :

E.coli derived recombinant. The protein contains the HAV structural proteins VP2-VP4 immunodominant regions.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >90% pure as determined by 10% PAGE (coomassie staining).

Protein Conc.:

1mg/ml in 10mM CBB, pH9.6, 0.1% SDS, 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with sera HAV-infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems.

Usage:

This material is offered by ProSpec-Tany TechnoGene for research laboratory or further manufacturing purposes.

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