Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HAV-226

Background:

HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.

Description :

The protein contains the HAV VP3 immunodominant regions, amino acids: (304-415). Hepatitis A Virus VP1 protein is purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HAV protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

2mg/ml. 1mg in 50mM Tris-HCl, pH8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Storage:

Recombinant HAV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HAV is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HAV-infected individuals

Application:

Recombinant HAV Antigen may be used in ELISA and Western blots, excellent for detection of HAV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Protein synonyms/aliases:

Genome polyprotein

Precursor's functional components:

Coat protein VP4 (P1A)
Coat protein VP2(P1B)
Coat protein VP3 (P1C)
Coat protein VP1 (P1D)
Picornain 2A(EC 3.4.22.29)
    (Core protein P2A)
Core protein P2B
Core protein P2C;Probable protein P3A
Probable protein P3B
Picornain 3C(EC 3.4.22.28)
    (Protease 3C)
    (P3C)
RNA-directed RNA polymerase(EC 2.7.7.48)
    (P3D) .

Protein Family:

Protein Domains:

Domains:
•IPR001676   Picornavirus capsid
•IPR000605   RNA helicase
•IPR000199   Peptidase C3A and C3B, picornaviral
•IPR007095   RNA-directed RNA polymerase, double-strand and positive-strand RNA virus
•IPR001205   RNA-directed RNA polymerase, P3D protein

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. A novel CD4+ T-helper lymphocyte epitope in the VP3 protein of hepatitis A virus.
J Med Virol 2004 Apr;72(4):525-32
2. Hepatitis A virus VP3 may activate serum response element associated transcription.
Scand J Gastroenterol 2003 Mar;38(3):307-13
3. Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design.
FEBS Lett 2003 Apr 10;540(1-3):133-40
4. Membrane fusion induced by a lipopeptidic epitope from VP3 capside protein of hepatitis A virus.
Luminescence 2001 Mar-Apr;16(2):135-43
5. Interaction study of peptide from VP3 capsid protein of hepatitis A virus through monolayers and fluorescence spectroscopy.
Luminescence 2001 Mar-Apr;16(2):103-7
6. Membrane fusion by an RGD-containing sequence from the core protein VP3 of hepatitis A virus and the RGA-analogue: implications for viral infection.
Biopolymers 2001 Jan;58(1):63-77

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中華民國95年06月06日更新