Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
HAV-227
Background:
HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.
Description :
The protein contains the HAV VP1 immunodominant regions, amino acids: (1323-1324 a.a.).
Hepatitis A Virus VP1 protein is purified by proprietary chromatographic techniques.
Purification method:
Purified by proprietary chromatographic technique.
Purity:
Protein is >90% pure as determined by 10% PAGE (coomassie staining).
Formulation:
7.5mg/ml. 1mg in 50mM Tris-HCl, pH8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
Storage:
Recombinant HAV is shipped at ambient temperature. Upon arrival, Store at -20C.
Stability:
Recombinant HAV is stable 5 years frozen, One month in solution at room temperature.
Specificity:
Immunoreactive with sera of HAV-infected individuals.
Application:
Recombinant HAV Antigen may be used in ELISA and Western blots, excellent for detection of HAV with minimal specificity problems.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Protein synonyms/aliases:
Genome polyprotein
Precursor's functional components:
Coat protein VP4 (P1A)
Coat protein VP2(P1B)
Coat protein VP3 (P1C)
Coat protein VP1 (P1D)
Picornain 2A(EC 3.4.22.29)
(Core protein P2A)
Core protein P2B
Core protein P2C;Probable protein P3A
Probable protein P3B
Picornain 3C(EC 3.4.22.28)
(Protease 3C)
(P3C)
RNA-directed RNA polymerase(EC 2.7.7.48)
(P3D) .
Protein Family:
Protein Domains:
Domains:
•IPR001676 Picornavirus capsid
•IPR000605 RNA helicase
•IPR000199 Peptidase C3A and C3B, picornaviral
•IPR007095 RNA-directed RNA polymerase, double-strand and positive-strand RNA virus
•IPR001205 RNA-directed RNA polymerase, P3D protein
Protein links: