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Recombinant Hepatitis A Virus VP1 (1323-1324 a.a.)

Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HAV-227

Background:

HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.

Description :

The protein contains the HAV VP1 immunodominant regions, amino acids: (1323-1324 a.a.). Hepatitis A Virus VP1 protein is purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >90% pure as determined by 10% PAGE (coomassie staining).

Formulation:

7.5mg/ml. 1mg in 50mM Tris-HCl, pH8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Storage:

Recombinant HAV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HAV is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HAV-infected individuals.

Application:

Recombinant HAV Antigen may be used in ELISA and Western blots, excellent for detection of HAV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.


Protein synonyms/aliases:

Genome polyprotein

Precursor's functional components:

Coat protein VP4 (P1A)
Coat protein VP2(P1B)
Coat protein VP3 (P1C)
Coat protein VP1 (P1D)
Picornain 2A(EC 3.4.22.29)
    (Core protein P2A)
Core protein P2B
Core protein P2C;Probable protein P3A
Probable protein P3B
Picornain 3C(EC 3.4.22.28)
    (Protease 3C)
    (P3C)
RNA-directed RNA polymerase(EC 2.7.7.48)
    (P3D) .

Protein Family:


Protein Domains:


Domains:
IPR001676   Picornavirus capsid
IPR000605   RNA helicase
IPR000199   Peptidase C3A and C3B, picornaviral
IPR007095   RNA-directed RNA polymerase, double-strand and positive-strand RNA virus
IPR001205   RNA-directed RNA polymerase, P3D protein

Protein links:

Recombinant Hepatitis A Virus VP1 (1323-1324 a.a.) protein domain

Recombinant Hepatitis A Virus VP1 (1323-1324 a.a.) protein family


Precursor- Protein structure and amino acid sequence:


Latest Publications:

1. Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design.
FEBS Lett 2003 Apr 10;540(1-3):133-40
2. Molecular evolution of hepatitis A virus: a new classification based on the complete VP1 protein.
J Virol 2002 Sep;76(18):9516-25
3. Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C.
J Virol 2002 Sep;76(17):8551-9
4. A proposed vestigial translation initiation motif in VP1 of hepatitis A virus.
Virus Res 2002 Jul;87(1):11-9
5. Maturation of the hepatitis A virus capsid protein VP1 is not dependent on processing by the 3Cpro proteinase.
J Virol 1999 Aug;73(8):6220-7
6. Hepatitis A virus capsid protein VP1 has a heterogeneous C terminus.
J Virol 1999 Jul;73(7):6015-23

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中華民國95年06月06日更新