Certificate of Analysis and Data Sheet

Source:
Escherichia Coli.

Catalog No.
PRO-435

Background:

The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo.

Description:

Recombinant Drosophila melanogaster GAGA-POZ produced in E.Coli is a single,non-glycosylated polypeptide chain containing 130 amino acids & having a molecular mass of 14 kDa.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) containing 10mM HEPES (pH7.4) and 25mM NaCl.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLLDLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQTVTKDDYTTH

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:Trl
Synonyms:Adf-2, GAGA, Nc70F, TFGAGA
ORFNames:CG33261

Protein synonyms/aliases:

Transcription factor GAGA (Trithorax-like protein)
    (GAGA factor)
    (GAF)
    (Adh transcription factor 2)
    (Neural conserved at 70F).

Protein Family:

Protein Domains:

Domains:
•IPR000210   BTB
•IPR013069   BTB/POZ
•IPR007087   Zinc finger, C2H2-type
•IPR000354   Involucrin repeat

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Repression by TTK69 of GAGA-mediated activation occurs in the absence of TTK69 binding to DNA and solely requires the contribution of the POZ/BTB domain of TTK69.
J Biol Chem 2004 Mar 12;279(11):9725-32
2. batman Interacts with polycomb and trithorax group genes and encodes a BTB/POZ protein that is included in a complex containing GAGA factor.
Mol Cell Biol 2003 Feb;23(4):1181-95
3. The N-terminal POZ domain of GAGA mediates the formation of oligomers that bind DNA with high affinity and specificity.
J Biol Chem 1999 Jun 4;274(23):16461-9
4. Co-operative DNA binding by GAGA transcription factor requires the conserved BTB/POZ domain and reorganizes promoter topology.
EMBO J 1999 Feb 1;18(3):698-708
5. Comparison of the GAGA factor genes of Drosophila melanogaster and Drosophila virilis reveals high conservation of GAGA factor structure beyond the BTB/POZ and DNA-binding domains.
Dev Genes Evol 1998 Oct;208(8):447-56

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中華民國95年06月06日更新