Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ALR-003

Background:

Description:

E.coli derived recombinant.
The protein contains the Der P1 epitope, amino acids (114-192 a.a).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein concentration:

1 mg/ml containing 10mM Tris-HCl, pH 6.5, 100mM Sodium Phosphate and 8M urea.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Application:

Use as an antigen in ELISA and Western Blots.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:DERP1

Protein synonyms/aliases:

Major mite fecal allergen Der p 1 precursor (EC 3.4.22.-)
    (Der p I).

Protein Family:

Belongs to the peptidase C1 family.

Protein Domains:

Domains:
•IPR000668   Peptidase C1A, papain C-terminal
•IPR013128   Peptidase C1A, papain

Related proteins:

•Recombinant Dermatophagoides F1 Protein - DerF1

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. A prospective study of Fel d1 and Der p1 exposure in infancy and childhood wheezing.
Am J Respir Crit Care Med 2004 Aug 1;170(3):273-8
2. Electrostatic charge characteristics of Der p1 allergen-carrying particles and the house dust mite dermatophagoides pteronyssinus.
Med Biol Eng Comput 1998 May;36(3):351-4
3. Purified Der p1 and p2 patch tests in patients with atopic dermatitis: evidence for both allergenicity and proteolytic irritancy.
Acta Derm Venereol 1998 Jul;78(4):241-3
4. Concentrations of cat (Fel d1), dog (Can f1) and mite (Der f1 and Der p1) allergens in the clothing and school environment of Swedish schoolchildren with and without pets at home.
Pediatr Allergy Immunol 1998 Feb;9(1):25-30
5. The allergen Der p1 induces NF-kappaB activation through interference with IkappaB alpha function in asthmatic bronchial epithelial cells.
Biochem Biophys Res Commun 1997 Jul 18;236(2):522-6
6. Is the dust mite allergen Der p1 a cysteine proteinase?
Biochem Soc Trans 1997 Feb;25(1):85S

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中華民國95年06月06日更新