Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CMV-215

Background:

The human cytomegalovirus UL99-encoded pp28 is a myristylated phosphoprotein that is a constituent of the virion. The pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane.

Description :

The protein contains the CMV-pp65 immunodominant regions, amino acids:(297-510). CMV-pp65 is purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant CMV-pp65 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25 mM Tris-Hcl pH 8.0, 1 mM EDTA and 50% glycerol.

Storage:

Recombinant CMV-pp65 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant CMV-pp65 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of CMV-infected individuals

Application:

Recombinant CMV-pp65 Antigen may be used in ELISA and Western blots, excellent for detection of CMV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:UL83

Protein synonyms/aliases:

65 kDa lower matrix phosphoprotein (PP65)
    (Tegument protein UL83).

Protein Family:

Belongs to the herpesviruses UL82 family.

Protein Domains:

Domains:
•IPR008649   Betaherpesvirus UL8283, N-terminal

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Molecular, biological, and in vivo characterization of the guinea pig cytomegalovirus (CMV) homologs of the human CMV matrix proteins pp71 (UL82) and pp65 (UL83).
J Virol 2004 Sep;78(18):9872-89
2. Human cytomegalovirus UL83-coded pp65 virion protein inhibits antiviral gene expression in infected cells.
Proc Natl Acad Sci U S A 2003 Sep 30;100(20):11439-44
3. Induction of HLA-G-restricted human cytomegalovirus pp65 (UL83)-specific cytotoxic T lymphocytes in HLA-G transgenic mice.
J Gen Virol 2003 Feb;84(Pt 2):307-17
4. Cross-presentation of human cytomegalovirus pp65 (UL83) to CD8+ T cells is regulated by virus-induced, soluble-mediator-dependent maturation of dendritic cells.
J Virol 2002 Jan;76(1):142-50
5. Incoming human cytomegalovirus pp65 (UL83) contained in apoptotic infected fibroblasts is cross-presented to CD8(+) T cells by dendritic cells.
J Virol 2000 Nov;74(21):10018-24
6. Immunogenicity evaluation of DNA vaccines that target guinea pig cytomegalovirus proteins glycoprotein B and UL83.
Viral Immunol 2000;13(2):155-67

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中華民國95年06月06日更新