Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CMV-216

Background:

The cytomegalovirus (CMV) phosphoprotein (PP) is a component of the tegument. It remains with the nucleocapsid fraction under conditions that remove most other tegument proteins from the virion, suggesting a direct and perhaps tight interaction with the capsid.

Description :

The protein contains the CMV-pp150 immunodominant regions, amino acids:(1011-1048). CMV-pp150 is purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant CMV-pp150 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25 mM Tris-Hcl pH 8.0, 1 mM EDTA and 50% glycerol.

Storage:

Recombinant CMV-pp150 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant CMV-pp150 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of CMV-infected individuals

Application:

Recombinant CMV-pp150 Antigen may be used in ELISA and Western blots, excellent for detection of CMV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:UL32

Protein synonyms/aliases:

Large structural phosphoprotein (pp150)
    (150 kDa matrixphosphoprotein)
    (Basic phosphoprotein)
    (BPP)
    (Tegument protein UL32).

Protein Family:

Protein Domains:

Domains:
•IPR010340   Herpesvirus large structural phosphoprotein UL32

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Characterization of host immunity to cytomegalovirus pp150 (UL32).
Hum Immunol 2005 Feb;66(2):116-26
2. Detection of cytomegalovirus late (pp150) antigen in peripheral blood leukocytes of patients with human immunodeficiency virus-related lymphoma.
J Infect Dis 2000 Jul;182(1):376-9
3. Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs.
J Infect Dis 2000 May;181(5):1537-46
4. Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in p lasma.
J Infect Dis 1999 May;179(5 ):1101-7
5. Human cytomegalovirus matrix protein PP150 is efficiently presented as one of target antigens for cytotoxic T lymphocyte recognition.
Chin Med J (Engl) 1997 May;110(5):397-400
6. Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims.
J Med Virol 1996 Oct;50(2):193-7

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中華民國95年06月06日更新