Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CMV-217

Background:

The glycoprotein B (gB) region of the human cytomegalovirus (H-CMV) is a major envelope glycoprotein that is a principal target of neutralizing antibodies and is known to stimulate the immune response of cytotoxic T lymphocytes. Human CMV is currently classified into four genotypes on the basis of the nucleotide sequence of the gB region.

Description :

E.coli derived recombinant. The artificial mosaic protein contains the CMV gB immunodominant regions,amino acids: (60-81) of gB protein repeated three times.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Protein Conc.:

1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with sera of CMV-infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

Usage:

This material is offered by ProSpec-Tany TechnoGene for research laboratory or further manufacturing purposes.

Latest Publications:

1. Isolation of a fusion protein containing the antigenic domain 1 of human cytomegalovirus glycoprotein B and its application in ELISA tests.
Biotechnol Lett 2006 Jan;28(2):73-7
2. Improved recovery of a fusion protein containing the antigenic domain 1 of the human cytomegalovirus glycoprotein B.
Biotechnol Lett 2005 Aug;27(16):1241-5
3. Antigenic domain 1 is required for oligomerization of human cytomegalovirus glycoprotein B.
J Virol 2005 Apr;79(7):4066-79
4. Site directed mutagenesis of the carboxyl terminus of human cytomegalovirus glycoprotein B leads to attenuation of viral growth in cell culture.
Arch Virol 2005 Mar;150(3):585-93
5. Mixed cytomegalovirus glycoprotein B genotypes in immunocompromised patients.
Clin Infect Dis 2004 Jul 15;39(2):155-61
6. Relationship between human cytomegalovirus glycoprotein B genotype and serum alanine aminotransferase elevation in infants.
Tohoku J Exp Med 2004 Aug;203(4):339-44