Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-310

Description :

Recombinant CDK8 (cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; cell division protein kinase 8; K35) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
Recombinant CDK-8 is expressed in E. coli as a full-length protein fused to a proprietary 16-Kd tag (a 6xHis tag is located at the very N-terminal end).

Formulation:

Recombinant CDK8 is supplied at 0.1mg/ml in a buffer containing 25 mM HEPES, pH 7.9, 125mM KCI, 0.5% TX-100, and 50% glycerol.

Storage:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Application:

Recombinant human CDK-8 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

Purity:

Greater than 90% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.