Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-280

Background:

The enzyme alanine aminotransferase (ALT) was previously known as serum glutamic pyruvic transaminase (SGPT). This enzyme also is correctly referred to as alanine transaminase. ALT is a cytoplasmic enzyme that catalyzes the transamination of alpha-ketoglutarate and L-alanine, forming glutamate and pyruvate.The highest activities of ALT are found in hepatocytes and striated (skeletal and cardiac) muscle cells. Therefore, increased serum ALT activity can accompany hepatocellular injury or necrosis of striated muscle. With cell injury or death, ALT (a "leakage" enzyme) escapes from the cytosol. Determination of ALT activity is a relatively sensitive indicator of hepatic damage in certain animal species and can help determine whether further diagnostic tests

Description :

Recombinant Alanine Aminotransferase produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 54,479 Dalton. Recombinant ALT is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile liquid formulation at a concentration of 1,430IU/ml.

Formulation:

The protein (1mg/ml) was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.

Stability:

rALT although stable at 10蚓 for 5 days, should be stored desiccated below -18蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec旧 Recombinant ALT is fully biologically active when compared to standard. The specific activity was found to be 957 IU/mg.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant ALT.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:GPT
Synonyms:AAT1, GPT1

Protein synonyms/aliases:

Alanine aminotransferase (EC 2.6.1.2)
    (Glutamic--pyruvic transaminase)
    (GPT)
    (Glutamic--alanine transaminase).

Protein Family:

Belongs to the class-I pyridoxal-phosphate-dependent

Protein Domains:

Domains:
•IPR004839   Aminotransferase, class I and II

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Relation between elevated serum alanine aminotransferase and metabolic syndrome in Korean adolescents.
Am J Clin Nutr 2005 Nov;82(5):1046-51
2. Can Peg-IFN alpha-2a plus ribavirin be used to treat patients with chronic hepatitis C and normal alanine aminotransferase levels?
Nat Clin Pract Gastroenterol Hepatol 2005 Mar;2(3):130-1
3. Association among serum ferritin, alanine aminotransferase levels, and metabolic syndrome in Korean postmenopausal women.
Metabolism 2005 N ov;54(11):1510-4
4. No independent association of alanine aminotransferase with risk of future type 2 diabetes in the Hoorn study.
Diabetes Care 2005 Nov;28(11):2812
5. Interleukin 6 and 12, alanine aminotransferase activity, and HCV viral load in children with chronic hepatitis C treated with interferon and ribavirin.
Inflammation 2004 Dec;28(6):319-25
6. Correlation between serum alanine aminotransferase activity and age: an inverted U curve pattern.
Am J Gastroenterol 2005 Oct;100(10):2201-4

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新