Certificate of Analysis and Data Sheet

Source:

Catalog No.
ENZ-267

Description :

Porcine enteropeptidase is a specific protease which cleaves after the lysine at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave a site followed by proline. If a fusion tag is located in the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. ProSpec旧 enterokinase is a highly purified enterokinase from porcine. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases.

Physical Appearance:

Sterile Liquid.

Formulation:

50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

Stability:

One year when stored at -20蚓, three weeks at room temperature.
Please avoid freeze-thaw cycles.

Unit definition:

One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25蚓 in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

Assay conditions:

Each reaction contains 3 ug of partially purified thioredoxin-NP-27 fusion protein and varying amounts of Porcine EK. Reactions were incubated at 25蚓 for 16 hours and analyzed on a Coomassie-stained 15% SDS gel.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of enteropeptidase.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PRSS7
Synonyms:ENTK

Protein synonyms/aliases:

Enteropeptidase precursor (EC 3.4.21.9)
    (Enterokinase)

Precursor's functional components:

Enteropeptidase non-catalytic mini chain
Enteropeptidase non-catalytic heavy chain
Enteropeptidase catalytic light chain .

Protein Family:

Belongs to the peptidase S1 family.

Protein Domains:

Domains:
•IPR000082   SEA
•IPR002172   Low density lipoprotein-receptor, class A
•IPR000859   CUB
•IPR000998   MAM
•IPR000859   CUB
•IPR002172   Low density lipoprotein-receptor, class A
•IPR001190   Speract/scavenger receptor
•IPR001254   Peptidase S1 and S6, chymotrypsin/Hap

Related proteins:

•Human Factor IX
•Recombinant Human Tissue Plasminogen Activator
•Recombinant Bovine Trypsin
•Recombinant Human Hepatocyte Growth Factor
•Recombinant Human Angiostatin Kringles 1-3

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Purification and further characterization of enteropeptidase from porcine duodenum.
J Biochem (Tokyo) 1999 May;125(5):947-51
2. Structural characterization of porcine enteropeptidase.
J Biol Chem 1994 Aug 5;269(31):19976-82
3. Identification of a mucosal form of enteropeptidase in triton X-100 extracts of porcine duodenal mucosa.
Biochim Biophys Acta 1977 Feb 9;480(2):450-60
4. On the catalytic and binding sites of porcine enteropeptidase.
Bi ochim Biophys Acta 1976 Dec 8;452(2):488-96
5. The effect of calcium ions on the hydrolysis of benzoylarginine ethyl ester by porcine enteropeptidase.
Biochim Biophys Acta 1976 Nov 8;452(1):161-4
6. The active site of porcine enteropeptidase. Selective inactivation of the peptidase activity.
Biochim Biophys Acta 1974 Jun 18;350(2):495-8

採購找生工，預算最輕鬆。

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中華民國95年06月06日更新