Certificate of Analysis and Data Sheet

Source:
Bovine Myocardium

Catalog No.
PRO-330

Description:

This protein consists of an 80 kDa large subunit and a 30 kDa small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose
CL-4B columns.

Physical Appearance:

Sterile Filtered colorless solution.

Formulation:

50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.

Stability:

m-Calpain although stable at 10⁰C for 1 week, should be stored desiccated below -18⁰C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by:
Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications:

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:
A- Dilute 1痞 of the antiserum against ?calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).
B- Add 1ug of protein to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Refrencesy:

Lane et al. (1992) A comparison of the intracellular distribution of ?calpain, mcalpain and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203:5-16.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:CAPN2
Synonyms:CANPL2

Protein synonyms/aliases:

Calpain-2 catalytic subunit (EC 3.4.22.53)
    (Calpain-2 large subunit)
    (Calcium-activated neutral proteinase 2)
    (CANP 2)
    (Calpain M-type)
    (M-calpain)
    (Millimolar-calpain)
    (Calpain large polypeptide L2).

Protein Family:

Belongs to the peptidase C2 family.

Protein Domains:

Domains:
•IPR001300   Peptidase C2, calpain
•IPR002048   Calcium-binding EF-hand

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Cyclin E both regulates and is regulated by calpain 2, a protease associated with metastatic breast cancer phenotype.
Cancer Res 2005 Dec 1;65(23):10700-8
2. Degradation of human aquaporin 0 by m-calpain.
FEBS Lett 2005 Dec 19;579(30):6745-8
3. Calpain may contribute to hereditary cataract formation in sheep.
Invest Ophthalmol Vis Sci 2005 Dec;46(12):4634-40
4. Effect of extremely low frequency magnetic fields on calpain activation.
Bioelectromagnetics 2006 Jan;27(1):43-50
5. Mitochondrial localization of mu-calpain.
Biochem Biophys Res Commun 2005 Dec 16;338(2):1241-7
6. Caspase and calpain function in cell death: bridging the gap between apoptosis and necrosis.
Ann Clin Biochem 2005 Nov;42(Pt 6):415-31

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新