Certificate of Analysis and Data Sheet Human Menopausal Gonadotropin (hMG)

Source:
Urine of post-menopausal women

Catalog No.
HOR-251

Description :

hMG is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women. The hMG is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized hMG in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized hMG although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution hMG should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological Activity:

ProSpec's hMG conforms the following activities: 100IU/mg of FSH and 100IU/mg LH.

Contaminants:

Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of hMG as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:

1. Ovulation induction in the new millennium: recombinant follicle-stimulating hormone versus human menopausal gonadotropin.
Gynecol Endocrinol 2005 Mar;20(3):161-9
2. Ovarian follicular development stimulated by leuprorelin acetate plus human menopausal gonadotropin in chimpanzees.
J Med Primatol 2005 Apr;34(2):73-85
3. Successful pregnancy, achieved by ovulation induction using a human menopausal gonadotropin low-dose step-up protocol in an infertile patient with Kallmann's syndrome.
J Obstet Gynaecol Res 2005 Apr;31(2):140-3
4. Reduction of dehydroepiandrosterone sulfate synthesis in women with polycystic ovary syndrome by human menopausal gonadotropin but not purified urinary follicle stimulating hormone: a comparative pilot study.
Gynecol Endocrinol 2004 Aug;19(2):69-78
5. The pregnancy rates with intrauterine insemination (IUI) in superovulated cycles employing different protocols (clomiphen citrate (CC), human menopausal gonadotropin (HMG) and HMG+CC) and in natural ovulatory cycle.
J Pak Med Assoc 2004 Oct;54(10):503-5
6. Human menopausal gonadotropin and recombinant follicle-stimulating hormone for controlled ovarian hyperstimulation in assisted reproductive cycles.
Fertil Steril 2003 Nov;80(5):1121-2