Source:
Catalog No.
HOR-284
Description : GLP-1 is a single, non-glycosylated, polypeptide chain containing 30 amino acids and having a molecular mass of 3298.7 Dalton.
GLP-1 is a incretion hormone, which has been shown to stimulate secretion of insulin in the presence of elevated blood glucose concentrations, but not during periods of low blood glucose concentrations (hypoglycemia). GLP-1 is released from gut endocrine cells in response to nutrient ingestion. The hormone is a physiologic mediator of satiety and regulates energy absorption and disposal.
GLP-1 is an insulinotropic hormone that has the ability to render pancreatic beta cells glucose-competent. It has been used in the therapy of type-2 diabetes.
GLP-1 has activities of cyokines, although it is not classified as such due its small size GLP-1 enhances cell differentiation, plays a role in tissue regeneration, and mediates cytoprotection.
GLP-1 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein (1mg/ml) was lyophilized with no additives.
Solubility: It is recommended to reconstitute the lyophilized GLP-1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Human GLP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Human GLP-1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be His-Ala-Glu-Gly-Thr.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: 1. Regulates Glucose levels rapidly
2. Reduces Insulin resistence
3. Reduces Glucagon
4. Reduces HbA1c
5. Stimulates beta cell grwoth which stimulates insulin production
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of GLP-1.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of GLP-1 as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:GCG
Glucagon precursor
Glicentin
Glicentin-related polypeptide(GRPP)
Oxyntomodulin (OXY)
(OXM)
Glucagon
Glucagon-like peptide 1(GLP-1)
Glucagon-like peptide 1(7-37)
(GLP-1(7-37))
Glucagon-likepeptide 1(7-36)
(GLP-1(7-36))
Glucagon-like peptide 2 (GLP-2) .
Belongs to the glucagon family.
Domains:
•IPR000532 Glucagon/GIP/secretin/VIP
•Recombinant Exendin-4
•Recombinant Human Glucagon Like Peptide-1 (7-36)
•Human Growth Hormone Releasing Hormone
