Certificate of Analysis and Data Sheet
Human Follicle Stimulating Hormone (HuFSH)
Source:
Urine of post-menopausal women
Catalog No.
HOR-249
Description :
Human FSH is a glycoprotein produced from urine of post-menopausal women and having a total molecular mass of 30,000 Dalton.
HuFSH is a heterodimeric hormone consisting of 92amino acids a chain and 111 amino acids b chain.
HuFSH induces maturation of Graafian follicles of the ovary, promotes development of germinal cells in males and activates cytosolic tyrosine kinase.
The HuFSH is purified by proprietary chromatographic techniques.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Solubility:
It is recommended to reconstitute the lyophilized FSH in sterile 18MΩ-cm
H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized FSH although stable at room temperature for 3 weeks, should be stored desiccated
below -18 C. Upon reconstitution FSH should be stored at 4 C between 2-7 days and for future use
below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Biological Activity:
ProSpec's HuFSH is biologically active compared to standard (IRP reference preparation 68/140).
The activity was found to be 626IU/mg.
Contaminants:
Less than: 0.1% hCG, 0.5%TSH, 0.5% LH, 0.5%GH and 0.5%Prl.
Free of HbsAg, antibodies to HIV and HCV.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard
solution of FSH as a Reference Standard.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Latest Publications:
1. A pen injection device for self-administration of recombinant follicle-stimulating hormone for fertility treatments.
Expert Rev Med Devices 2005 Jan;2(1):27-32
2. Serum luteinizing hormone in patients undergoing ovarian stimulation with gonadotropin-releasing hormone antagonists and recombinant follicle-stimulating hormone and its relationship with cycle outcome.
Fertil Steril 2005 Nov;84(5):1529-32
3. Serum inhibin B and follicle-stimulating hormone levels as markers in the evaluation of azoospermic men: a comparison.
Andrologia 2005 Oct;37(5):173-9
4. A reference preparation of buffalo pituitary follicle stimulating hormone using lectin affinity chromatography.
Prep Biochem Biotechnol 2005;35(4):331-45
5. Sperm concentration and normal sperm morphology decrease and follicle-stimulating hormone level increases with age.
BJU Int 2005 Nov;96(7):1087-91
6. High frequency of Y chromosome microdeletions in idiopathic azoospermic men with high follicle-stimulating hormone levels.
J Urol 2005 Nov;174(5):1943