Certificate of Analysis and Data Sheet
Human Chorionic Gonadotropin (hCG)
Source:
Urine of post-menopausal women
Catalog No.
HOR-250
Description :
hCG is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women having a total molecular mass of 36,700 Dalton. hCG consists of 237 amino acids, chain-92 amino acids and chain-145 amino acids.
The hCG is purified by proprietary chromatographic techniques.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Solubility:
It is recommended to reconstitute the lyophilized hCG in sterile 18MΩ-cm
H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated
below -18 C. Upon reconstitution hCG should be stored at 4 C between 2-7 days and for future use
below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Biological Activity:
ProSpec's hCG is biologically active compared to standard (IRP reference preparation 75/551).
The activity was found to be 5000 IU/mg.
Contaminants:
Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard
solution of hCG as a Reference Standard.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Latest Publications:
1. Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma.
World J Gastroenterol 2005 Dec 21;11(47):7401-4
2. EFFECTS OF HUMAN CHORIONIC GONADOTROPIN (hCG) ON PITUITARY PROLACTIN (PRL) IN THE LIZARD, UROMASTIX HARDWICKII.
Pak J Pharm Sci 2004 Jul;17(2):21-7
3. LUTEINIZING HORMONE RECEPTORS TRANSLOCATE TO PLASMA MEMBRANE MICRODOMAINS FOLLOWING BINDING OF HUMAN CHORIONIC GONADOTROPIN.
Endocrinology 2006 Jan 12;
4. Treatment with human chorionic gonadotropin for PADAM: A preliminary report.
Aging Male 2005 Sep-Dec;8(3):175-9
5. Choriocarcinoma with negative urinary and serum beta human chorionic gonadotropin (betaHCG)--a case report.
Indian J Med Sci 2005 Dec;59(12):538-41
6. [Metastatic tumor of spermatic c ord with elevation of serum human chorionic gonadotropin beta-subunit]
Nippon Hinyokika Gakkai Zasshi 2005 Nov;96(7):714-6