Certificate of Analysis and Data Sheet

Source:

Catalog No.
HOR-275

Description :

Thymosin B4 is a 43 amino acid peptide which is regarded as the main intracellular G-actin sequestering peptide. Extracellular Thymosin b4 may contribute to physiological processes such as angiogenesis, wound healing, and regulation of inflammation. Thymosin b4 has an a.a. sequence of Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Thymosin beta4 in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Thymosin b4 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Thymosin b4 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Thymosin b4.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Thymosin beta4 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:TMSB4Y
Synonyms:TB4Y

Protein synonyms/aliases:

Thymosin beta-4, Y-chromosomal.

Protein Family:

Belongs to the thymosin beta family.

Protein Domains:

Domains:
•IPR001152   Thymosin beta-4

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar.
Histochem Cell Biol 2005 Sep;124(3-4):207-13
2. A comparative analysis of RNA targeting strategies in the thymosin beta 4 gene.
J Mol Biol 2004 Sep 24;342(4):1069-76
3. Selective nonsteroidal anti-inflammatory drugs induce thymosin beta-4 and alter actin cytoskeletal organization in human colorectal cancer cells.
J Pharmacol Exp Ther 2004 Dec;311(3):885-91
4. Overexpression of the thymosin beta-4 gene is associated with increased invasion of SW480 colon carcinoma cells and the distant metastasis of human colorectal carcinoma.
Oncogene 2004 Aug 26;23(39):6666-71
5. Assignment of the thymosin beta 4 X/Y chromosome (TMSB4X/Y) gene to tammar wallaby chromosome 5p by fluorescence in situ hybridisation.
Cytogenet Genome Res 2003;103(1-2):203F
6. Up-regulation of thymosin beta 4 gene expression in experimentally-induced uterine adenomyosis in mice.
In Vivo 2003 Nov-Dec;17(6):561-5

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中華民國95年06月06日更新