Certificate of Analysis and Data Sheet

Source:

Catalog No.
HOR-241

Description :

Thymopentin, also known as TP-5 is a synthetic derivative of thymopoietin, a thymic hormone, and has immunoregulatory properties. Thymopentin has a molecular formula of C₃₀H₄₉N₉O₉
H-Arg-Lys-Asp-Val-Tyr-OH having a Mw of 679.8.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Thymopentin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Thymopentin although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution TP-5 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of TP-5.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Thymopentin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:

1. Trypsin-catalyzed kinetically controlled synthesis of a precursor dipeptide of thymopentin in organic solvents, using a free amino acid as nucleophile.
Prep Biochem Biotechnol 2004 Feb;34(1):45-56
2. Increased radiation toxicity by enhanced apoptotic clearance of HL-60 cells in the presence of the pentapeptide thymopentin, which selectively binds to apoptotic cells.
Mutat Res 2004 Mar 14;558(1-2):19-26
3. [Stability evaluation of thymopentin in preparation process]
Sichuan Da Xue Xue Bao Yi Xue Ban 2003 Apr;34(2):292-4
4. [Therapeutic effects of Lamivudine in combination with Thymopentin on chronic hepatitis B]
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Dec;16(4):385-7
5. [Modulating effects of thymopentin on the immune system of severely burned patients]
Zhonghua Shao Shang Za Zhi 2001 Oct;17(5):298-300
6. Stimulation of nonspecific resistance by thymopentin and its analogs against Leishmania donovani infection in hamsters.
Peptides 1999 Nov;20(11):1381-3