Certificate of Analysis and Data Sheet

Source:
Bacterium Streptomyces avidinii

Catalog No.
PRO-283

Description:

Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240 kDa and containg 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth.
The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.

Physical Appearance:

Sterile Filtered lyophilized powder.

Formulation:

1mM MgCl2, 0.1mM ZnCl2, 30 mM triethanolamine pH 7.6.

Solubility:

It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H²O + 0.1M NaCl not less than 100ug/ml or more than 10mg/ml solutions.

Stability:

Streptavidin although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Application:

Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.

Activity:

Greater than 13 U/mg, 1 unit binds 1ug biotin.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Streptavidin.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Comparison study of streptavidin-coated microtitration plates.
J Immunol Methods 2006 Jan 20;308(1-2):203-15
2. PNA-DNA hybridization study using labeled streptavidin by voltammetry and surface plasmon fluorescence spectroscopy.
Anal Chem 2006 Jan 15;78(2):470-6
3. Pretargeted Radioimmunotherapy with a Single-Chain Antibody/Streptavidin Construct and Radiolabeled DOTA-Biotin: Strategies for Reduction of the Renal Dose.
J Nucl Med 2006 Jan;47(1):140-9
4. Crystallographic Analysis of a Full-length Streptavidin with Its C-terminal Polypeptide Bound in the Biotin Binding Site.
J Mol Biol 2006 Feb 24;356(3):738-45
5. Enhanced antigen-specific antibody and cytokine responses when targeting antigen to human FcGAMMA receptor type I using an anti-human FcGAMMA receptor type I-streptavidin fusion protein in an adjuvant-free system.
Immunol Invest 2005;34(4):417-29
6. Solid lipid nanoparticles can effectively bind DNA, streptavidin and biotinylated ligands.
Eur J Pharm Biopharm 2006 Feb;62(2):155-62