Certificate of Analysis and Data Sheet

Source:

Catalog No.
HOR-274

Description :

Sincalide also called CCK-8 has a molecular formula of C₄₉H₆₂N₁₀O₁₆S₃ H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH₂ having a Mw of 1143.29. Sincalide corresponds to the C-terminal octapeptide of cholecystokinin (CCK) which acts on receptors within the gallbladder wall causing it to contract. Cholecystokinin (CCK) is a hormone originally isolated from porcine intestinal mucosa and described as a linear 33-amino acid peptide containing a sulfated tyrosine, which is essential for its biological activity. It has been found in mammals in both the digestive tract and the central nervous system. Among its multiple biological functions, this hormone stimulates pancreatic exocrine secretion, gallbladder contraction, and intestine motility and may also act as a neurotransmitter/neuromodulator in the central nervous system.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The Sincalide was lyophilized from a concentrated (1mg/ml) solution with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Sincalide in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Sincalide although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Sincalide should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Mass Spectral Anaylsis {MALDI-TOF exhibits correct Mw}.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CCK

Protein synonyms/aliases:

Cholecystokinins precursor (CCK)

Precursor's functional components:

Cholecystokinin 58(CCK58)
Cholecystokinin 39 (CCK39)
Cholecystokinin 33 (CCK33);Cholecystokinin 12 (CCK12)
Cholecystokinin 8 (CCK8) .

Protein Family:

Belongs to the gastrin/cholecystokinin family.

Protein Domains:

Domains:
•IPR013152   Gastrin/cholecystokinin
•IPR001651   Gastrin/cholecystokinin peptide hormone

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Sincalide in patients with parenteral nutrition-associated gallbladder disease.
Ann Pharmacother 2004 Nov;38(11):1942-5
2. Compounded versus proprietary sincalide for evaluation of gallbladder ejection fraction.
Ann Pharmacother 2004 Mar;38(3):428-32
3. Responsibility for sincalide injection obtained from compounding pharmacies.
Am J Health Syst Pharm 2002 Jul 1;59(13):1295-6
4. L-365,260 reversed effect of sincalide against morphine on electrical and mechanical activities of rat duodenum in vitro.
Acta Pharmacol Sin 2002 Jul;23(7):582-6
5. The unavailability of sincalide.
J Nucl Med 2002 May;43(5):35N; author reply 35N
6. Normal values for sincalide cholescintigraphy: comparison of two methods.
Radiology 2001 Nov;221(2):404-10

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中華民國95年06月06日更新