Certificate of Analysis and Data Sheet Human Secretin

Source:

Catalog No.
HOR-273

Description :

Human Secretin stimulates the secretion of bicarbonate by the pancreas and inhibits the production of gastrin and acid production in the stomach. It also potentiates the release of digestive enzymes from the pancreas triggered by cholecystokinin.
Secretin has a molecular formula of C₁₃₀H₂₂₀N₄₄O₄₀ a.a. sequence of -His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg- Glu-Gly-Ala-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Gly-Leu-Val-NH2 and having a Mw of 3039.45.

Fields of Applications:

Diagnosis of pancreatic dysfunction.
Diagnosis of the presence of a gastrinoma.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Human Secretin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Human Secretin although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Human Secretin should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Secretin.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Human Secretin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:SCT

Protein synonyms/aliases:

Secretin precursor.

Protein Family:

Belongs to the glucagon family.

Protein Domains:

Domains:
•IPR000532 Glucagon/GIP/secretin/VIP

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Glucagon- and secretin-related peptides differentially alter ocular growth and the development of form-deprivation myopia in chicks.
Invest Ophthalmol Vis Sci 2005 Nov;46(11):3932-42
2. Double doses of secretin contribute to diagnosis of Zollinger-Ellison syndrome in secretin and selective arterial secretion injection tests--a case report.
Dig Dis Sci 2005 Nov;50(11):2034-6
3. Prospective evaluation of the accuracy of the intraductal secretin stimulation test in the diagnosis of chronic pancreatitis.
Clin Gastroenterol Hepatol 2005 Jul;3(7):695-9
4. [Secretin]
Nippon Rinsho 2005 Aug;63 Suppl 8:512-6
5. Intravenous secretin for autism spectrum disorder.
Cochrane Database Syst Rev 2005 Jul 20;(3):CD003495
6. Partial reversal of phencyclidine-induced impairment of prepulse inhibition by secretin.
Biol Psychiatry 2005 Jul 1;58(1):67-73

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中華民國95年06月06日更新