Certificate of Analysis and Data Sheet

Source:
Rabbit

Catalog No.
ANT-157

Background:

The spike (S) glycoprotein of coronaviruses mediates viral entry into host cells.Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. It is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted HR-N and HR-C, that form coiled-coil structures within the ectodomain of the protein. Previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes.

Description:

Full-length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139KDa band is detected.

Antigen:

The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 1124-1140 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).

Isotype:

Rabbit IgG

Formulation:

0.5ug/痞 PBS containing 0.2% gelatin and 0.05% sodium azide.

Storage:

Store at 4C, stable for 6 months. For long-term storage, store at -20C

Applications

Western blot SARS detection.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Biochem Biophys Res Commun 2005 Dec 23;338(3):1654-60
2. False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome.
J Med Virol 2005 Nov;77(3):331-6
3. A serological survey on neutralizing antibody titer of SARS convalescent sera.
J Med Virol 2005 Oct;77(2):147-50
4. First external quality assurance of antibody diagnostic for SAR S-new coronavirus.
J Clin Virol 2005 Sep;34(1):22-5
5. Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity.
Clin Exp Immunol 2005 Sep;141(3):500-8
6. Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.
Immunol Lett 2005 Sep 15;100(2):177-81