Certificate of Analysis and Data Sheet

Source:
Rabbit

Catalog No.
ANT-182

Background:

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family.

Antigen:

The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 238-254 of the Anthrax PA protein (~83 kD).

Isotype:

Rabbit IgG

Formulation:

200痞 of unpurified sera in PBS containing 0.2% gelatin and 0.05% sodium azide.

Storage:

Store at 4C, stable for 6 months. For long-term storage, store at -20C

Applications

Western blot detection of Anthrax PA protein.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Plant-based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge.
Infect Immun 2005 Dec;73(12):8266-74
2. Immunogenicity of recombinant protective antigen and efficacy against aerosol challenge with anthrax.
Infect Immun 2005 Sep;73(9):5978-87
3. Anthrax biosensor, protective antigen ion channel asymmetric blockade.
J Biol Chem 2005 Oct 7;280(40):34056-62
4. A high-affinity monoclonal antibody to anthrax protective antigen passively protects rabbits before and after aerosolized Bacillus anthracis spore challenge.
Infect Immun 2005 Feb;73(2):795-802
5. Late treatment with a protective antigen-directed monoclonal antibody improves hemodynamic function and survival in a lethal toxin-infused rat model of anthrax sepsis.
J Infect Dis 2005 Feb 1;191(3):422-34
6. [Monoclonal antibodies to B.anthracis protective antigen are capable to neutralize and to enhance the anthrax lethal toxin action in vitro]
Mol Gen Mikrobiol Virusol 2004;(3):21-6