Certificate of Analysis and Data Sheet

Source:
Propionibacterium shermanii

Catalog No.
ENZ-266

Background:

PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.

Description :

Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD (monomer)

Physical Appearance:

Lyophilized powder

Formulation:

Contains polyphosphates and phosphate buffer salts.

Stability:

One year when stored at -20蚓, three weeks at room temperature.
Please avoid freeze-thaw cycles.

Required cofactors:

The reaction requires Mg²⁺.

Unit definition:

1 Unit of activity = the amount of PPK required to convert 1 micromole ADP to ATP per minute at 30C and pH 7.4, in phosphate buffer, using polyphosphate as phosphate donor. (The reaction is reversible.)

Biological Activity:

1-3 U per mg protein

Applications:

The PPK reaction provides an inexpensive means of recycling ATP consumed in enzymatic phosphorylation reactions. This has potential for use in enzymatic oligosaccharide synthesis, which requires phosphorylated sugar intermediates.

E.C number:

2.7.4.1

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Diverse phenotypes resulting from polyphosphate kinase gene (ppk1) inactivation in different strains of Helicobacter pylori.
J Bacteriol 2005 Nov;187(22):7687-95
2. A novel ATP regeneration system using polyphosphate-AMP phosphotransferase and polyphosphate kinase.
J Biosci Bioeng 2001;91(6):557-63
3. Polyphosphate kinase regulates error-prone replication by DNA polymerase IV in Escherichia coli.
Mol Microbiol 2005 Aug;57(3):751-61
4. Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.
EMBO Rep 2005 Jul;6(7):681-7
5. Polyphosphate kinase protects Salmonella enterica from weak organic acid stress.
J Bacteriol 2005 May;187(9):3088-99
6. Polyphosphate kinase: a new colonization factor of Helicobacter pylori.
FEMS Microbiol Lett 2005 Feb 1;243(1):45-50