Certificate of Analysis and Data Sheet

Source:
Propionibacterium shermanii

Catalog No.
ENZ-265

Background:

Polyphosphate Glucokinase catalyzes the phosphorylation of glucose using either inorganic polyphosphate or ATP as the phosphoryl donor. The phosphorylation of glucose plays a significant role in the creation of intermediates for oligosaccharide synthesis

Description :

Polyphosphate Glucokinase purified circa 100 fold . Free of all polyphosphate kinase activity. Native MW = 63 kD, subunit MW = 30kD,Isoelectric point = 5.8.

Physical Appearance:

Lyophilized powder

Formulation:

Contains polyphosphates and phosphate buffer salts.

Stability:

One year when stored at -20蚓, three weeks at room temperature.
Please avoid freeze-thaw cycles.

Required cofactors:

The reaction requires Mg²⁺.

Enzymological properties:

T(optimum) = 50蚓
pH (optimum) 9-10

Unit definition:

1 Unit of activity = the amount of PPGK required to convert 1 痠ole glucose to glucose-6-phosphate per minute at 30蚓 and pH 7.4, in phosphate buffer using polyphosphate as phosphate donor.

Biological Activity:

10-40 U per mg protein

E.C number :

2.7.1.63

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Polyphosphate glucokinase.
Prog Mol Subcell Biol 1999;23:101-25
2. Kinetic mechanisms of polyphosphate glucokinase from Mycobacterium tuberculosis.
Biochemistry 1996 Jul 30;35(30):9772-81
3. Initial rate and equilibrium isotope exchange studies on the ATP-dependent activity of polyphosphate Glucokinase from Propionibacterium shermanii.
Biochemistry 1996 May 28;35(21):6777-85
4. Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis.
J Biol Chem 1996 Mar 1;271(9):4909-15
5. Determination of endopolyphosphatase using polyphosphate glucokinase.
Anal Biochem 1993 Jul;212(1):194-205
6. Glucose determination using immobilized polyphosphate glucokinase.
Anal Biochem 1991 Sep 2;197(2):326-32