Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-287

Background:

L-Asparaginase is an enzyme that depletes L-Asparagine "an important nutrient for cancer cells" resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli.,which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukaemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease.

Description :

L-Asparaginase produced from E.Coli containing 303 amino acids and having a molecular mass of 31731 Dalton.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The enzyme was lyophilized with no additives.

Purity:

Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE stained Coomassie blue gel.

Stability:

Two years when stored at ?0蚓, 2 weeks at 4蚓

Unit definition:

One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 ?mol of ammonia per minute at pH 7.3 and 37蚓.

Specific Activity:

250,000IU/mg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ybiK
OrderedLocusNames:b0828

Protein synonyms/aliases:

Putative L-asparaginase precursor (EC 3.5.1.1)
    (L-asparagineamidohydrolase).

Protein Family:

Belongs to the asparaginase 2 family.

Protein Domains:

Domains:
•IPR000246   Peptidase T2, asparaginase 2

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. L-asparaginase as a marker of chemotherapy dose modification in children with acute lymphoblastic leukemia.
Cancer 2005 Dec 15;104(12):2858-61
2. [Temporary effective treatment with L-asparaginase for a patient with refractory nasal NK/T-cell lymphoma]
Gan To Kagaku Ryoho 2005 Nov;32(12):1993-6
3. Does L-asparaginase influence efficacy or toxicity when added to a standard CHOP protocol for dogs with lymphoma?
J Vet Intern Med 2005 Sep-Oct;19(5):732-6
4. Long-term results of a randomized trial on extended use of high dose L-asparaginase for standard risk childhood acute lymphoblastic leukemia.
J Clin Oncol 2005 Oct 1;23(28):7161-7
5. Selective apoptosis of natural killer-cell tumours by l-asparaginase.
Br J Haematol 2005 Sep;130(6):860-8
6. [Purification and properties of recombinant Erwinia carotovora L-asparaginase expressed in E.coli cells]
Biomed Khim 2003 Sep-Oct;49(5):502-7

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中華民國95年06月06日更新