Certificate of Analysis and Data Sheet

Source:
Human urine

Catalog No.
PRO-413

Description:

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from 0.05 M Acetate buffer pH 4.5.

Reconstitution:

0.1M Acetate buffer, pH 4.5 is recommended.

Stability:

Store at -20蚓.
Minimum shelf life 2 years.

Applications:

ELISA, Western blotting.

References:

?Nicklin M.J.H. and Barrett A.J.: Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984)
?Lindahl P., Abrahamson M., Bjork L.: Interaction of recombinant human cystatin c with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996)
?Page M.K. et al.: Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000)
?Ono S. et al.: Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. Acta Neurol Scand. 102, 47-52 (2000) Recombinant Mouse GST-Bax
?Price Ch.P., Finney H.: Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000)

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:CST3

Protein synonyms/aliases:

Cystatin C precursor (Neuroendocrine basic polypeptide)
    (Gamma-trace)
    (Post-gamma-globulin).

Protein Family:

Belongs to the cystatin family.

Protein Domains:

Domains:
•IPR000010   Proteinase inhibitor I25, cystatin

Protein links:

Precursor- Protein structure and amino acid sequence:

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中華民國95年06月06日更新