Certificate of Analysis and Data Sheet

Source:
Human alpha-fetoprotein

Catalog No.
PRO-406

Background :

Description :

AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

100痞 purified human Alpha Fetoprotein protein at 0.1mg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20⁰C to -80⁰C. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Applications:

?ELISA
?Inhibition Assays
?Western Blotting

Charactweization:

On SDS-PAGE commassie blue stained gel, the purified protein shows a band at 70kDa.

Activity:

Not known.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:AFP

Protein synonyms/aliases:

Alpha-fetoprotein precursor (Alpha-fetoglobulin)
    (Alpha-1-fetoprotein).

Protein Family:

Belongs to the ALB/AFP/VDB family.

Protein Domains:

Domains:
•IPR000264   Serum albumin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Role of blood AFP mRNA and tumor grade in the preoperative prognostic evaluation of patients with hepatocellular carcinoma.
World J Gastroenterol 2005 Nov 28;11(44):6920-5
2. [A case of AFP-producing gastric cancer with multiple liver metastasis responding to TS-1 and cisplatin combination chemotherapy]
Nippon Shokakibyo Gakkai Zasshi 2005 Dec;102(12):1523-8
3. AFP benefits from medical editing clerkship.
Am Fam Physician 2005 Nov 15;72(10):1946
4. Solitary AFP- and PIVKA-II-producing hepatoid gastric cancer with giant lymph node metastasis.
Hepatogastroenterology 2005 Nov-Dec;52(66):1930-2
5. Utility of FDG-PET for investigating unexplained serum AFP elevation in patients with suspected hepatocellular carcinoma recurrence.
Anticancer Res 2005 Nov-Dec;25(6C):4719-25
6. Prevention of N-methyl-N-nitrosourea-induced breast cancer by alpha-fetoprotein (AFP)-derived peptide, a peptide derived from the active site of AFP.
Clin Cancer Res 2005 Dec 1;11(23):8512-20

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中華民國95年06月06日更新