Certificate of Analysis and Data Sheet
Source:
Human
Catalog No.
PRO-325
Background:
Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71.
Transferrin appears to be indispensable for most cells growing in tissue culture.
It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis.
Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity
Transferrin has been identified also as one of the factors promoting the clonal growth of murine granulocyte and macrophage precursors cultured in vitro under serum-free conditions.
Description :
Iron saturated Human Holo Transferrin is a glycoprotein of approximately 77 kDa containing 679 amino acids.
Transferrin is purified by proprietary chromatographic techniques.
Physical Appearance:
Sterile Filtered lyophilized (freeze-dried) powder.
Formulation:
Transferrin (1mg/ml) was lyophilized with no additives.
Solubility:
It is recommended to reconstitute the lyophilized Transferrin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18蚓. Upon reconstitution HTF should be stored at 4蚓 between 2-7 days and for future use below -18蚓.
Please prevent freeze-thaw cycles.
Please avoid freeze-thaw cycles.
Purity:
Greater than 97.0% as determined by:
(a) Cellulose Acetate.
Human virus test:
FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis.
Viral inactivation by pasteurization (60蚓 for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented.
The purification process has also been found to remove significant additional quantities of virus.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Latest Publications:
1. Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor.
FEBS J 2005 Dec;272(24):6344-53
2. Analysis of carbohydrate-deficient transferrin: comparative evaluation of turbidimetric immunoassay, capillary zone electrophoresis, and HPLC.
Clin Chem 2005 Dec;51(12):2368-71
3. Cell-mediated LDL oxidation: the impact of transition metals and transferrin.
Biochem Biophys Res Commun 2005 Dec 23;338(3):1617-24
4. Bicarbonate inhibits the growth of Staphylococcus epidermidis in platelet concentrates by lowering the level of non-transferrin-bound iron.
Transfusion 2005 Nov;45(11):1768-73
5. A Japanese family with ferroportin disease caused by a novel mutation of SLC40A1 gene: hyperferritinemia associated with a relatively low transferrin saturation of iron.
Intern Med 2005 Sep;44(9):990-3
6. A ferrireductase fills the gap in the transferrin cycle.
Nat Genet 2005 Nov;37(11):1159-60