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Eledoisin

Certificate of Analysis and Data Sheet

Source:

Catalog No.
PRO-281

Description:

A protein that is formed in the venom gland of several species of octopuses and is used as a vasodilator and a contraction agent of extravascular smooth muscle Its molecular weight is 1188.4 having an amino acid sequence of Glp-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2 and a molecular formula of C54H85N13O15S

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Eledoisin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Eledoisin although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Eledoisin should be stored at 40C between 2-7 days and for future use below -180C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Eledoisin.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.


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Protein Family:


Protein Domains:


Domains:

Protein links:

Eledoisin protein domain

Eledoisin protein family


Precursor- Protein structure and amino acid sequence:


Latest Publications:

1. Eledoisin and Kassinin, but not Enterokassinin, stimulate ion transport in frog skin.
Peptides 2004 Nov;25(11):1971-5
2. Solution structure of the tachykinin peptide eledoisin.
Biophys J 2003 Jan;84(1):655-64
3. Reaction medium engineering in enzymatic peptide fragment condensation: synthesis of eledoisin and LH-RH.
Bioorg Med Chem 1998 Jul;6(7):891-901
4. A determination of the solution conformation of the nonmammali an tachykinin eledoisin by NMR and CD spectroscopy.
Biochemistry 1994 Jun 7;33(22):6802-11
5. Effect of angiotensin II and eledoisin on cholinergic neurons in rat hippocampus.
Neuroreport 1992 Jan;3(1):36-8
6. Radioiodinated substance P, neurokinin A, and eledoisin bind predominantly in NK1 receptors in guinea pig lung.
Mol Pharmacol 1992 Jan;41(1):147-53

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中華民國95年06月06日更新