Certificate of Analysis and Data Sheet

Source:

Catalog No.
HOR-270

Description :

Desmopressin also called ADH (Anti-Diuretic Hormone) has a molecular formula of C46H64N14O12S2 , Mpr-Tyr-Phe-Gln-Asn-Cys-Pro-D-Arg-Gly-NH2 having a Mw of 1069.23 is the first vasopressin analog with a very high and very specific antidiuretic effect, has been widely used for different therapeutic purposes and is believed to be partly responsible for the formation of memories learning and memory processes. Desmopressin increases urine concentration and decreases urine production. Desmopressin is used to prevent and control excessive thirst, urination, and dehydration.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Desmopressin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized DDAVP although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Desmopressin should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of DDAVP.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of DDAVP as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:

1. Recurrence of Cushing's disease preceded by the reappearance of ACTH and cortisol responses to desmopressin test.
Pituitary 2004;7(3):183-8
2. [The role of the renin-aldosterone system in osmoregulatory reactions of healthy subjects in response to desmopressin]
Fiziol Cheloveka 2005 Sep-Oct;31(5):110-6
3. Lack of desmopressin (DDAVP) response in men with hemophilia A following liver transplantation.
J Thromb Haemost 2005 Oct;3(10):2259-63
4. Retention test Homburg to monitor the adhesive properties of von Willebrand factor after substitution or desmopressin therapy.
Semin Thromb Hemost 2005;31(4):441-8
5. [Acute hyponatremia and Ogilvie's syndrome due to accidental desmopressin overdosage]
Gastroenterol Hepatol 2005 Aug-Sep;28(7):435-6
6. Desmopressin is a safe drug for the treatment of enuresis.
Scand J Urol Nephrol 2005;39(4):308-12