Certificate of Analysis and Data Sheet

Source:
Dog Heart.

Catalog No.
PRO-410

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-HCL and 1mM EGTA.

Solubility:

It is recommended to reconstitute the lyophilized Calsequestrin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Calsequestrin although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Calsequestrin should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by:
Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications:

Immunoblots.
Absorption.
Immunohistochemistry

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Transcriptional regulation of sarcoplasmic reticulum Ca(2+)-ATPase and calsequestrin genes in cardiac myocytes.
J Muscle Res Cell Motil 2004;25(8):609-10
2. Effects of drugs with muscle-related side effects and affinity for calsequestrin on the calcium regulatory function of sarcoplasmic reticulum microsomes.
Mol Pharmacol 2005 Dec;68(6):1708-15
3. Targeted inhibition of beta-adrenergic receptor kinase-1-associated phosphoinositide-3 kinase activity preserves beta-adrenergic receptor signaling and prolongs survival in heart failure induced by calsequestrin overexpression.
J Am Coll Cardiol 2005 Jun 7;45(11):1862-70
4. Effects of calsequestrin over-expression on excitation-contraction coupling in isolated rabbit cardiomyocytes.
Cardiovasc Res 2005 Sep 1;67(4):667-77
5. Regulation of ryanodine receptors by calsequestrin: effect of high luminal Ca2+ and phosphorylation.
Biophys J 2005 May;88(5):3444-54
6. Modulation of sarcoplasmic reticulum calcium release by calsequestrin in cardiac myocytes.
Biol Res 2004;37(4):603-7