Certificate of Analysis and Data Sheet

Source:
Human Eryhtrocytes

Catalog No.
PRO-282

Physical Appearance:

Sterile Filtered colorless solution.

Formulation:

50mM imidazole-HCl, 0.2mM EGTA and 1mM DTT pH-7.4. Tris-HCL and 1mM EGTA.

Stability:

Calpastatin although stable at 10⁰C for 1 week, should be stored desiccated below -18⁰C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by: Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications:

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure:
A-Dilute 1 痞 of the antiserum against Calpastatin in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).
B- Add 1ug of protein to 1ml of the diluted antibody solution and mix well.
C-Incubate for at least 6 hours in the cold.
D-Apply to tissue-sections and incubate for 3 days.
E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Refrencesy:

Lane et al. (1992) A comparison of the intracellular distribution of ?calpain, mcalpain and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203:5-16.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle.
J Anim Sci 2006 Feb;84(2):291-9
2. Expression and immunolocalization of the calpain-calpastatin system during parthenogenetic activation and fertilization in the rat egg.
Reproduction 2006 Jan;131(1):35-43
3. Activation of calpains, calpastatin and spectrin cleavage in the brain during the pathology of fatal murine cerebral malaria.
Neurochem Int 2006 Jan;48(2):108-13
4. [RA-related autoantibodies (anti-calpastatin, anti-gp130-RAPS, and anti-FRP antibodies)]
Nippon Rinsho 2005 Jul;63 Suppl 7:449-52
5. Involvement of calpain-calpastatin in cigarette smoke-induced inhibition of lung endothelial nitric oxide synthase.
Am J Respir Cell Mol Biol 2005 Nov;33(5):513-20
6. An assessment of extraction and assay techniques for quantification of calpain and calpastatin from small tissue samples.
J Anim Sci 2005 Sep;83(9):2182-8