Certificate of Analysis and Data Sheet

Catalog No.
PRO-415

Source

Description :

Botulinum Toxin Type-A blocks neuromuscular transmission by binding to acceptor sites on motor nerve terminals, entering the nerve terminals and inhibiting the release of acetylcholine. The inhibition occurs as the neurotoxin cleaves SNAP-25, a protein integral to the successful docking and release of acetylcholine from vesicles situated within nerve endings. When injected intramusculary at therapeutic doses it produces partial chemical denervation of the muscle resulting in a localized reduction in muscle activity. In addition, the muscle may atrophy, axonal sprouting may accur, and acetylcholine receptors may develop. There is an evedence that reinnervation of the muscle pay occur, thus slowly reversing muscle denervation produced by the BTX-A.
Botulinum toxin type A is the highly purified protein consiting of a 50 kDa light chain and a 100 kDa heavy chain linked by disulfide bonding and is produced from fermentation of Hall Starin Clostridium Botulinum type A grown in a medium containing casein hydrolysate, glucose and yeast extract. It is purified from the culture solution by dialysis and a series of acid precipitations to a complex consisting of the neurotoxin and several accessory proteins.

Manufacturing flow chart:

Strain -->activation--> cultivating -->strain removing and filtering--> isoelectric point precipitation -->Toxin extract -->enzyme treatment and concentrate--> chromatography -->crystallize -->Dilute and bottling -->freezing and drying packing.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Each 100IU contain 0.5mg HAS, 30mg dextran, 30mg mannitol and 0.9mg NaCl.

Stability:

Lyophilized Botulinum Toxin Type-A although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Botulinum Toxin Type-A should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of BTX-A.

Unit Definition:

1 Unit of BTX-A corresponds to 1 LD50 calculated median intraperitoneal dose in mice.

Specific Activity:

20 Units per nanogram of neurotoxin protein complex.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:botA
Synonyms:atx, bna

Protein synonyms/aliases:

Botulinum neurotoxin type A precursor (EC 3.4.24.69)
    (BoNT/A)
    (Bontoxilysin A)
    (BOTOX)

Precursor's functional components:

Botulinum neurotoxin A light-chain
Botulinum neurotoxin A heavy-chain .

Protein Family:

Belongs to the peptidase M27 family.

Protein Domains:

Domains:
•IPR000395   Peptidase M27, bontoxilysin
•IPR012500   Clostridium neurotoxin, translocation
•IPR012928   Clostridium neurotoxin, receptor binding N-terminal

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

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中華民國95年06月06日更新