Certificate of Analysis and Data Sheet

Source:
Hen's egg white.

Catalog No.
PRO-500

Description:

Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin.
Avidin is purified by affinity chromatographic techniques.
The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).

Physical Appearance:

Sterile Filtered lyophilized powder.

Solubility:

It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H₂O not less than 100ug/ml or more than 10mg/ml. solutions

Stability:

Avidin although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Application:

Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.

Activity:

Greater than 11 U/mg, 1 unit binds 1ug biotin.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Avidin.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. [Development of an antigen detection dot blot assay for the diagnosis of human onchocerciasis based on the biotin-avidin binding system]
Bull Soc Pathol Exot 2005 Sep;98(3):177-81
2. Application of avidin-biotin technology for the characterization of a model hapten-protein conjugate.
J Immunoassay Immunochem 2005;26(4):285-93
3. Distribution and residual activity of two insecticidal proteins, avidin and aprotinin, expressed in transgenic tobacco plants, in the bodies and frass of Spodoptera litura larvae following feeding.
J Insect Physiol 2005 Oct;51(10):1117-26
4. Avidin plate assay system for enzymatic characterization of a histone lysine methyltransferase.
Anal Biochem 2005 Jul 15;342(2):287-91
5. Avidin expressed in transgenic rice confers resistance to the stored-product insect pests Tribolium confusum and Sitotroga cerealella.
Biosci Biotechnol Biochem 2005 May;69(5):966-71
6. Improved pretargeted delivery of radiolabelled hapten to human tumour xenograft in mice by avidin chase of circulating bispecific antibody.
Eur J Nucl Med Mol Imaging 2005 Aug;32(8):901-9