Certificate of Analysis and Data Sheet

Source:

Catalog No.
HOR-239

Description :

Atosiban also called ADH (Anti-Diuretic Hormone) has a molecular formula of C₄₃H₆₇N₁₁O₁₂S₂, Mpr-Tyr(OEt)-Ile-Thr-Asn-Cys-Pro-Orn-Gly-NH2 having a Mw of 994.2 is the first oxytocin antagonist to be specifically developed for the treatment of preterm labour. Atosiban has a specific mode of action, inhibiting oxytocin-induced uterine contractions lry blocking oxytocin receptors in the uterus. Extensive clinical investigations have shown Atosiban to be at least as effective as current tocolytic agents. In addition, due to its novel and specific mode of action, Atosiban has a markedly improved maternal side effects profile compared with conventional therapies.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Atosiban in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Atosiban although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Atosiban should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Atosiban.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of Atosiban as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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