Certificate of Analysis and Data Sheet

Source:
Bovine lung

Catalog No.
PRO-285

Background:

Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. It is present in blood and in most tissues, with a high concentration in lung, inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins

Description :

Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids arranged in a single polypeptide chain, cross-linked by three disulfide bridges and having a molecular mass of 6512 Daltons. Aprotinin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Aprotinin (1mg/ml) was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the Aprotinin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Aprotinin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Aprotinin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological activity:

Prospec's Aprotinin activity was found to be 6x10⁶ IU/mg.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Aprotinin.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Protein synonyms/aliases:

Pancreatic trypsin inhibitor precursor (Basic protease inhibitor)
    (BPI)
    (BPTI)
    (Aprotinin).

Protein Family:

Protein Domains:

Domains:
•IPR002223   Proteinase inhibitor I2, Kunitz metazoa

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The risk associated with aprotinin in cardiac surgery.
N Engl J Med 2006 Jan 26;354(4):353-65
2. Aprotinin in major orthopedic surgery: a systematic review of randomized controlled trials.
Anesth Analg 2005 Dec;101(6):1602-7
3. Randomized controlled trials of aprotinin in cardiac surgery: could clinical equipoise have stopped the bleeding?
Clin Trials 2005;2(3):218-29; discussion 229-32
4. Kidney-specific proteins in patients receiving aprotinin at high- and low-dose regimens during coronary artery bypass graft with cardiopulmonary bypass.
Eur J Anaesthesiol 2005 Sep;22(9):666-71
5. Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion .
J Thorac Cardiovasc Surg 2005 Sep;130(3):662-9
6. The effects of aprotinin and tranexamic acid on thrombin generation and fibrinolytic response after cardiac surgery.
Acta Anaesthesiol Scand 2005 Oct;49(9):1272-9

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中華民國95年06月06日更新