Plant Growth Regulator

6-[4-Hydroxy-3-methyl-but-2-enylamino] purine
C₁₀H₁₃N₅O
M.W.: 219.24
Cytokinin growth regulator
Mixed isomers, 90% trans isomer
Balance primarily cis isomer.

Store at -20℃

Plant Growth Regulator

[9-(b-D-Ribofuranosyl)-trans-zeatin]
C₁₅H₂₁N₅O₅
M.W.: 351.4
Mixed isomers: trans isomer 97%
Cis-isomer: >2%
ZR was used for plant regeneration from tomato, Brassica nigna and Vigna sublobata protoplast.
ZR was used for direct and efficient regeneration from leaf explants of potato. From all cytokinins tested, ZR produced the maximum number of shoots per explant. Yadav NR and Sticklen MB. PCR 14: 645-647(1995)
Bhadra stock et al., PCR 14: 175-179(1994). Hossain M et al., PCTOC 42: 141-146(1995). Narasimhulu SM et al., PCTOC 32(1): 35-39(1993).

Store at -20℃

Zeocin is a glycopeptide antibiotic of the bleomycin family. It is active in vivo against most bacteria (including E. coli), eukaryotic microorganisms (i.e. yeasts), plant cells, and animal cells. 

USAGE:
Liquid Zeocin™ is ready for cell culture.
The concentration of Zeocin required for selection of transformants is pH and salt concentration dependent. The higher the pH and the lower the salt concentration, the better is Zeocin activity.
Selection of common E. coli strains is achieved at 25 μg/mL zeocin in Low Salt LB Agar medium adjusted to pH 7.5 .
Selection of animal cells: 100 to 750 μg/mL depending on the cell line.
Selection of plant cells: 50 to 250 μg/mL depending on species and media.
Selection of yeasts: Depends on species and media. S. cerevisiae Sh ble transformants are selected at 50 μg/mL in YEPD at pH 7.0

Store at -20℃