For Electrophoresis

Dissolved in ddH₂O. 0.45um filter filtrated.
Ready to use. Easy to use.

Store at Room Temp.

Molecular Biology Buffer

3-[ Tris(hydroxymethyl)methylamino]-1-propane sulfonic acid. C₇H₁₇NO₆S
M.W.: 243.28. 
Assay (Titration): 99.0%min.
pH ( 0.1M in water, 25℃): 4.5~6.0
Solubility (5% Soln. in water): Clear and Complete
pKa(25℃): 8.35±0.2

Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research. 

Store at Room Temp.

Terrific Broth

pH: 7.2±0.2

TB Dry is specifically designed to give high yields of low copy plasmids and extremely high yields of high copy plasmids. The media is pH stabilized to increase bacterial growth and produce higher yields of plasmid DNA.

It is formulated to increase cell density by as much as 4-7 times over cultures grown in standard LB Broth.

Prepare 1 liter soln. with 47.6g TB Dry Powder and add 8mL of Glycerol before autoclaving. After autoclaving, mix immediately while it is still hot. The media may appear cloudy at first but will become clear when cooled to room temperature.

Store at Room Temp.

For Electrophoresis

Dissolved in ddH₂O. 0.45um filter filtrated.
Ready to use. Easy to use.

Store at Room Temp.

For Electrophoresis, Easy to use.

One pack contains 85.225g of TBE Powder containing the following components:
TRIS: 54g
Boric Acid: 27.5g
EDTA: 3.725g

Dissolve 1 pack  in ddH₂O to make 5L of 1X TBE Buffer.

Store at Room Temp.

10xTBS Buffer ; 10x Rinse Buffer

Description
TBS is isotonic and non-toxic to cells and is suitable for molecular biology. 10xTBS Buffer is commonly used as substance diluent or as wash buffer in immunoassays such as ELISA. It is used in immuno-histochemical staining when the background is high and for diluting alkaline phosphatase or peroxidase-conjugated antibodies in Western blotting.

The 10×TBS Buffer is a pH stabilizing solution used for western blot and ELISA procedures. It is an optimal formulation of salts and pH stabilizers which enable washing without disruption of antibody-antigen binding interactions.
10×TBS Buffer contains 1.51M Sodium Chloride and 0.20M Tris/Tris-HCl.

Application
10xTBS Buffer is used in biochemical and molecular biology applications.
1.    Wash buffer in immunoassays
2.    Anibody diluent in Western blotting
3.    Sample diluent in in vitro diagnostics
4.    Immuno-histochemical staining
5.    In Situ hybridization

Features
Formulated from analytical grade chemicals;
Isotonic, non-toxic buffer;
Guaranteed reproducibility;
Ready to use in minutes.

Usage
Dilute to a 1xTBS Buffer with ddH2O before use

Store at Room Temp.

For Electrophoresis Gel Preperation

Tetramethylethylenediamine , TMEDA
C₆H₁₆N₂
M.W.: 116.21
Assay: 99.9% min.
pH: 8.0

TEMED was introduced as an enhancer of the polymersitation (cross-linking) of acrylamide and bisacrylamide in gel electrophoresis. It catalysis the formation of free radicals of the initiator of the polymerisation, ammonium persulfate. If gels are degased to remove oxygen, add the TEMED after degasing.

Working conc.: 50uL/100mL

Needles, H.L. (1970) Anal. Biochem. 35, 533-537 Effect of solution components on large-pore polyacrylamide gel formation.
Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.
Gomes, A.V. & Barnes, J.A. (1998) Anal. Biochem. 260, 106-108 Gel electrophoresis of mini gels.

Store at 4℃

Harmful! Handle under a chemical fume hood. 

Harmful, Highly flammable, Corrosive

Molecular Biology Buffer

2-[Tris-(hydroxymethyl)methylamino]-1-ethane sulfonic acid
C₆H₁₅NO₆S
M.W.: 229.25
Assay: 99.0%min.

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.

Store at Room Temp.

D-Thymidine 3',5'-diphosphate disodium salt

C10H14N2Na2O11P2

M.W.: 446.15

Purity (TLC): min 95%

off-white solid

Melting point: >213℃ (dec.)

Store at -20℃.

Molecular Biology Buffer

N-[ Tris-(Hydroxymethyl)-Methyl Glycine
C₆H₁₃NO₅
M.W.: 179.09
Assay (Titration): 99.0%min.
Moisture: 0.62%
pKa (25℃): 7.96
pH (1% Soln. in water ):5.39
A₂₆₀ (0.1M in water): 0.0068
A₂₈₀ (0.1M in water): 0.0042
White Crystal Powder

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.

Store at Room Temp.

Molecular Biology Buffer, DNA Electrophoresis

Tris-(hydroxymethyl)- aminomethane, 
C₄H₁₁NO₃
M.W.: 121.14
Assay (By Titration): 99.99% min.
Heavy metals: 5ppm max.
pH (5% Soln. in water): 10.0-11.5
Solubility (40% Soln. in water): Clear and Colorless
White Crystalline Powder.

Tris is the most commonly used buffer in biological research. One of the most important applications is the use as an electrophoresis buffer for polyacrylamide and agarose gel electrophoresis, respectively. Tris should not be used at pH values under pH 7.2 or above pH 9.0. The pH value of a Tris buffer strongly depends on the temperature. Therefore, Tris buffers should be prepared at the temperature where it is used.

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.

Store at Room Temp.

Irritant

SDS-PAGE Electrophoresis Buffer

Description
5x Tris-Glycine Buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 110 volts for mini vertical gel electrophoresis units.

Application
5x Tris-Glycine Buffer is used for running Protein samples for SDS PAGE analysis on Polyacrylamide gels.
It is also used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets.

Reconstitution
Dilution of the 5x Tris-Glycine Buffer produces a 1× running buffer containing 25 mM Tris, 251 mM Glycine and 0.1% SDS.

Recommended Use
Add 1/5 volume of 5×Tris-Glycine Buffer to 4/5 volume of ddH2O

Store at Room Temp.

Molecular Biology Buffer

Tris-(Hydroxymethyl)-aminomethane hydrochloride
C₄H₁₂NO₃Cl
M.W.: 157.60
Assay (Titration): 99%min.
Heavy metals(as Pb): <5ppm
pH(0.5M in water): 3.5-5.0
A₂₆₀ (0.5M in water): 0.015
A₂₈₀ (0.5M in water): 0.010

Store at Room Temp.

M.W.: 648.85
Assay: 99.0%
Appearance: Liquid, clear to slightly hazy, colorless to light yellow

Triton X-100 is a nonionic detergent, 100% active ingredient, which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered a comparatively mild non-denaturing detergent and is reported in numerous references. It does absorb in the ultraviolet region of the spectrum, so it can interfere with protein quantitation by absorption at A280nm. A number of polymeric resins have been used to remove X-100 from solution, including Amberlite hydrophobic XAD resins and Rezorian A161 cartridges.

For lysing cells, typically about 0.1% X-100 solution in water will be sufficient, and even up to 0.5% concentrations will usually not harm most enzymes being isolated. Many enzymes remain active in the presence of X-100; for example, Proteinase K, remains active in 1% (w/w) solutions of X-100. Triton X-100 can be detected in the parts per million range by spectrophotometric measurement of the concentration of a Triton-ammonium-cobalt-thiocyanate complex. Interfering substances in this assay have been discussed.

Store at Room Temp.

For Microbiology Culture

Total nitrogen TN: 12.9%
pH (2% solution): 6.9
Loss on drying: 4.8%
a-Amino nitrogen AN: 4.1%
Residue on ignition: 11.8% 
Chloride (NaCl): 0.3%
Total aerobic microbial count: 172/g

Tryptone is a component of many bacterial growth rmedia (e. g. LB-Medium, TB-Medium, YT-Medium etc.). It is hygroscopic - Store in a dry place.

Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York. 

Store at Room Temp. Keep Dry!

Specific density d20 4: 1.095
Saponification value (KOH mg/g): 40-55
Hydroxyl value (KOH mg/g): 90-110
Appearance: Clear, yellow to yellow-green viscous liquid

TWEEN 20 is a nonionic detergent widely used in biochemical applications.
(1) It is a frequently used member of the polysorbate family. These have been used as emulsifying agents for the preparation of stable oil-inwater emulsions.
(2) It has been used in preextraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
(3) It has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%.
(4) It can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%.

TWEEN 20 is miscible in water (100 mg/ml), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate; and is practically insoluble in liquid paraffin and fixed oils.

Store at Room Temp.