For Electrophoresis

Dodecyl sulfate sodium salt
C₁₂H₂₅NaO₄S
Measurements of molecular weights by gel electrophoresis.
M.W.: 288.38
Assay: 95% min.
Free alcohols: < 0.4%
Homologs: < 1% 
NaCl: < 1% 
Na₂SO₄: <2.5%

SDS is an anionic detergent which binds nearly to all water-soluble proteins. This binding leads to a conformational change and allows to separate proteins by size. SDS should be used at a concentration of 0.1 % in the gel and in the electrophoresis buffer.

Laemmli, U.K. (1970) Nature 227, 680-685 Cleavage of structural proteins during the assembly of the head of Bacteriophage T4.
Harewood, K. & Wolff III, J.S. (1973) Anal. Biochem. 55, 573-581 A rapid electrophoretic procedure for the detection of SDS-released Oncorna-viral RNA using polyacrylamide-agarose gels.
Nielsen, T.B. & Reynolds, J.A. (1978) Methods Enzymol. 48, 3-10

Store at Room Temp. 

Harmful. Handle the powder in a chemical fume hood. Do not breathe dust. 

Harmful, Irritant

Acetic acid, sodium salt
Molecular Formula:  C2H3O2Na
M.W.=82.03
99.0 to 101.0%

Buffer, suitable for electrophoresis buffers

Store cool and dry. Keep away from direct sunlight or strong incandescent light.

NaCl
M.W.: 58.44
Assay: 99.8% min.
FInsoluble matter: < 0.005%
Loss on Drying: < 0.2%
Bromide (Br): < 0.02%
Iodide (I): < 0.002%
Sulphate (SO₄): < 0.002%
Nitrate (NO₃): < 0.002%
Nitride (N): < 0.001%
Magnesium (Mg): < 0.002%
Potassium (K): < 0.02%
Calcium (Ca): < 0.007%
Iron (Fe): < 3ppm
Arsenic (As): < 0.5ppm
Heavy Metals (as Pb): < 5ppm
pH (20℃, 1M in water): 5.0-8.0(lit.)
Melting Point: 801℃(lit.)
Solubility (20℃, 1M in water):  clear, colorless

Iglewski, B.H., Sadoff, J.C., In gradient elution techniques. Meth. Enzymol. 60, 780, (1979), Fieser 6,285
Hathaway, G.M. Br. Pharm. 60, 495, (1979), Aldrich MSDS 1, 1608:C / Corp MSDS 1 (2), 3135:D / RegBook 1 (3), 3319:F / Sax 6, 2419
Hathaway, G.M. Br. Pharm. 60, 495, (1979), For the preparation of isotonic solutions. Br. Pharm., 742, (1963)
Leckband, D.E. , Role of calcium in the adhesion and fusion of bilayers. Biochemistry 32, 1127, (1993)
Higson, S.P.J., Vadgama, P. Electroanalysis 6, 431, (1994)

Store at Room Temp.

WB Stripping buffer

Nitrocellulose and PVDF membranes that have been probed by Western blotting procedures and detected by chemiluminescent or other nonprecipitating substrates may be used to detect tubulin, actin and other stable-expression proteins as a consult or to compare with the other proteins.

Western Blot Stripping Buffer provides a robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane. Compared with a new SDS-PAGE, it is much simpler and easier and can avoid the error causing by new loading.

Advantages:
Stripping high-affinity antibody;
Effective use of samples that are available in limited amounts;
Multiple times of re-probes (~20 times) ;
Comparison of images obtained with different antibodies in the same blot ;
Confirmation of results with the same or different antibodies ;
It is simply more economical and less time consuming to reuse blots for re-probing and mass spectrometry.

Procedures:
1. Wash the blot in distilled water for 5 minutes after Chemiluminescent detection of Western.
2. Place the blot in Western Blot Stripping Buffer. Place container onto a shaker/rotator and wash for 5 minutes. Use a sufficient volume to ensure that the blot is completely wetted.
3. Remove the blot from the Western Blot Stripping Buffer and wash in enough distilled water for 5 minutes on a shaker/rotator. Never let the blot dry.
4. Blocking and subsequent procedures could be performed.

Stable at room temperature for 1 year. Note! Take safety precautions when operating because Western Blot Stripping Buffer is mildly corrosive.