Protein Experiment, Membrane Protein Isolation

n-Octyl-b-D-Glucopyranoside
C₁₄H₂₈O₆
M.W.: 292.37

Non-ionic detergent for the solubilization and isolation of membrane proteins

Octylglucoside is one of the most commonly used detergents for membrane protein studies. It has many good properties, allowing the isolation of functional, active proteins. 

Working conc.: 30mM, fresh preparation.

Baron, C. & Thompson, T.E. (1975) Biochim. Biophys. Acta 382, 276-285 Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine.
Stubbs, G.W. et al. (1976) Biochim. Biophys. Acta 425, 46-56 Alkyl glucosides as effective solubilizing agents for bovine rhodopsin: a comparison with several commonly used detergents.

Store at -20℃

Enzymatic Colorimetric Reagent, for b-D-Galactosidase

o-Nitrophenyl-ß-D-galacto-pyranoside
C₁₂H₁₅NO₈
M.W.: 301.25
Assay: 99% min.

 ONPG is one of the major substrates in β-galactosidase enzyme assays. The cleavage of ONPG releases the yellow dye 4-nitrophenyl and absorption is measured at 405 - 420 nm by spectrophotometry.

Wallenfels, K. (1962) Methods Enzymol. 5, 212-219 β-Galactosidase: Assay methods. 

Store at -20℃