Reduced Form Electron Donor

C₂₁H₂₇N₇Na₂O₁₄P₂
M.W.: 709.40
Assay.: 98% min.
Reduced form Disodium Salt

Composition of the buffer for the reduction of oxaloacetate: 30 mM potassium phosphate pH 7.5, 0.5 mM oxaloacetate, 0.1 mM NADH, 20 mM KCl, 5 mM MgCl₂.
Composition of the buffer for the reduction of pyruvate: 100 mM MOPS pH 6.1, 10 mM Na-pyruvate, 100 mM Na₂CO₃, 0.1 mM NADH, 5 mM MgCl₂. 

Kobayashi, K. et al. (1989) J. Biol. Chem. 264, 3200-3205
Structure and properties of malic enzyme from Bacillus stearothermophilus. 

Store at -20℃

Reduced Form Electron Donor

Nicotinamide Adenine Dinucleotide Phosphate 
C₂₁H₂₆N₇Na₄O₁₇P₃
M.W.: 833.35 
Reduced form Tetrasodium Salt 

Store at -20℃

Enzymatic Colorimetric Reagent for N-Acetylneuraminic Acid Aldolase

n-Acetylneuraminic acid, synthetic sialic acid
C₁₁H₁₉NO₉
M.W.: 309.27
Substrate for N-Acetylneuraminic acid aldolase. Hygroscopic.

Store at -20℃

Enzymatic Colorimetric Reagent for Alkaline phosphotase

3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)4,4'-diyl]bis-(2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium] Chloride, Nitro Blue Tetrazolium
C₄₀H₃₀N₁₀O₆Cl₂
M.W.: 817.64
Assay: 99% min.
Loss on Drying: 8.5g/100g
Sulfated Ash: 0.02g/100g, TLC Single spot
Yellow Crystalline Powder

The most popular application of NBT is the determination of the activity of alkaline phosphatase in combination with BCIP. This reaction is based on the oxidation of indoxyl to the insoluble, blue indigo by NBT. A particularly sensitive and clean material to give blue-violet formazan at 530nm.

NBT Stock conc.: 50 mg/mL in 70% dimethylformamide, store in dark
X-Phos (BCIP) Stock conc.: 50 mg/mL in 100% dimethylformamide, store in dark

Prepare the BCIP/NBT developing solution just before use as follows:
i. Add 33 μL of the NBT solution to 5 mL of AP buffer and mix well. ii. Add 16.5 μL of the stock solution of BCIP. Mix again. Protect the solution from strong light and use it within 1 hour. 

Store at 4℃. Protected form light!

C12H22O(CH2CH2O)nH
Density: 1.05-1.07g/ml
Solubility(5%,water):clear
Sulfate:<0.003%

Nonidet P40 is used for solubilizing membrane proteins during isolation of membrane-protein complexes.

Membrane proteins are highly sensitive towards peroxides and carbonyl compounds. While proteins are oxidized by peroxides, the Schiff’s base which is formed during the reaction with carbonyl compounds can possibly influence the protein function. Also salts are disturbing the isolation of membrane bound proteins. Detergents of the polyoxoethylene type could contaminants of peroxides and carbonyl compounds and salts. Peroxide formation is catalyzed by light. Nonidet P40 has been purified to reduce levels of unwanted peroxides or carbonyl compounds or salts.

Store at 18-25℃ for 5 years.