D-(+)Maltose, monohydrate

Biochemistry reagent grade

C12H22O11 • H2O, MW=360.32

pH(50 g/L, 20 oC): 4.5~6.5
Solubility (H2O, 20 oC): 50 mg/mL
Specific rotary power: +128°～+132°

Store at RT

Molecular Biology Buffer

2-(N-Morpholino)EthaneSulfonic acid
C6H13NO4S
M.W.: 195.2
99% Min.
Enzyme free
Loss on Drying: <1.0%
Melting Point: >300℃
pKa (25℃): 6.10±0.2
pH (0.5M in water, 20℃): 2.5-4.0

MES is component of e.g. the DNase buffer, does not interfere with the Folin protein assay.

Good, N.E. et al. (1966) Biochemistry 5, 467-477
Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310
Hydrogen ion buffers for biological research.

Store at RT

Molecular Biology Buffer

2-(N-Morpholino)EthaneSulfonic Acid Monohydrate C6H13NO4S•H2O
M.W.: 213.26

MES is component of e.g. the DNase buffer, does not interfere with the Folin protein assay.

Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68 Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.

Store at Room Temp.

H-Met-OH

C5H11NO2S
M.W.: 149.2
Appearance：White crystalline powder
Assay(dry basis)：99% min

Storage
Cool dry place (<25°C)

For PCR Use, Autoclaved

Autoclaved. Suitable for overlaying aqueous reactions like PCR.

Store at Room Temp.

Molecular Biology Buffer, RNA Eletrophoresis

3-(N-Morpholino)PropaneSulfonic acid
C₇H₁₅NO₄S
M.W.: 209.26
99% Min.
Loss on Drying: <1.0%
pKa (25℃): 7.15±0.2
pH(10.0% in water 20℃): 3.0-5.0

MOPS interferes with the Folin (Lowry) protein assay, but not the Biuret protein assay. It doesn't bind metal ions and decomposes partially, when autoclaved in the presence of glucose.

Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310
Hydrogen ion buffers for biological research.

Store at Room Temp.

Culture Medium for Lactobacilli

52.4g for 1L Soln. Components follow:
Peptone: 10.0g
Beef Extract: 8.0g
Yeast Extract: 4.0g
Glucose: 20.0g
Diammonium hydrogen citrate: 2.0g
Dipotassium hydrogen phosphate: 2.0g
Sodium Acetate: 5.0g
Magnesium Sulfate: 0.2g
Manganese Sulfate: 0.04g
Tween 80: 1.0g
PH: 5.7±0.2

Preparation Procedure:
1. Dissolve 5.24g of the medium in 100mL of purified water.
2. Heat with frequent agitation to boiling to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.

Quality control & Expected Cultural Response
1. Inoculate 10-100 Lactobacillus directly into MRS Broth.
2. Incubate broth tubes at 35°C for 3 days, or at 30°C for 5 days in an anaerobic atmosphere.
3. Growth of Lactobacillus spp. appears turbid.

Avoid direct sunshine. Keep Dry.

Enzymatic Colorimetric Reagent

Thiazolyl Blue Tetrazolium Bromide
3-(4,5-Dimethyl-Thiazolyl)-2,5-Diphenyl-2H-Tetrazolium Bromide
C₁₈H₁₆N₅SBr
M.W.: 414.32
99% Min.
Red-violet formazan with max. at 565nm
Reagent grade yellow crystals
Loss on Drying: 0.011%
Sulfated Ash: 0.02%
Melting Point: 195-196℃

Mosmann, T. (1983) J. Immunol. Methods 65, 55-63
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays.
Gerlier, D. & Thomasset, N. (1986) J. Immunol. Methods 94, 57-63
Use of MTT colorimetric assay to measure cell activation.
Hansen, M.B. et al. (1989) J. Immunol. Methods 119, 203-210
Re-examination and further development of a precise and rapid dye method for measuring cell growth / cell kill.

Store at 4℃

Enzymatic Colorimetric Reagent for GUS Detection

4-MethylUmbelliferyl-Beta-D-Glucuronide
C₁₆H₁₆O₉
M.W.: 352.30
98.5% Min.

Fluorescent substrate of GUS encoded by gusA gene.

Mead, J.A.R. et al. (1955) Biochem. J. 61, 569-574
The biosynthesis of the glucuronides of Umbelliferone and 4-Methylumbelliferone and their use in fluorimetric determination of Beta-Glucuronidase.
Langley, S.D. et al. (1983) J. Biol. Chem. 258, 7416-7424
Genetic variant of Beta-Glucuronidase in Drosophila melanogaster.

Store at -20℃