Molecular Biology Buffer

3-(CyclohexylAmino)-1-PropaneSulfonic Acid
C₉H₁₉NO₃S
M.W.: 221.32
Assay (from N): 99% Min.
pKa (25ºC): 10.2-10.6
Loss on Drying: 1% Max.
A₂₆₀ (0.1M in water): 0.05 Max.
A₂₈₀ (0.1M in water): 0.02 Max.

CAPS is an alkaline buffer (pH 10-11, concentration 10-50mM, pH adjustment by NaOH) for protein transfer after SDS polyacrylamide gel electrophoresis on nitrocellulose filters.

Store at Room Temp.

DNA/RNA Experiment, For Density Gradient Centrifugation

CsCl
MW:168.36
99.9% Min.
A₂₆₀ (50% w/w H₂O): 0.023
A₂₈₀ (50% w/w H₂O): 0.018

For density gradient centrifugation, No enzymes, Low heavy metals.

Store at RT and keep dry.

Molecular Biology Buffer, A Nondenaturing Zwitterionic Detergent for Membrane Biochemistry

3-[(3-Cholamidopropyl)Dimethylamino]-1-Propanesulfonate
C₃₂H₅₈N₂O₇S
M.W.: 614.88
Assay (from N): 99.94%
TLC: one spot
Loss on Drying: 0.96%
Solubility (10% in water, 25ºC): Clear and colorless

CHAPS allows the isolation of many proteins in their native state, without loosing much of its activity. Protein-protein interactions can be interrupted more efficiently as with Triton X-100 or sodium cholate.

CHAPS interferes with the Lowry protein assay.

Working Conc.: 1-13mM

Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-6370
A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis.

Store at 4ºC

Molecular Biology Buffer

2-(CycloHexylamino)-Ethane Sulfonic Acid
C₈H₁₇NO₃S
M.W.: 207.29
Assay (Titration): 99.62%
A₂₆₀ (0.1M in water): 0.0078
A₂₈₀ (0.1M in water): 0.0061

CHES interferes with the Lowry assay.

Ellis, K.J. & Morrison, J.F. (1982) Methods Enzymol. 87, 405-426
Buffers of constant ionic strength for studying pH-dependent processes.

Store at Room Temp.

For amplification of DNA fragments by PCR, label the DNA fragment with radioactive isotope, biotin etc., development of PCR diagnostic kit and T-A cloning.

5U/uL
Include 10X reaction buffer and MgCl₂ solution.
Highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities.
Purity: 95%Min by SDS-PAGE.

Combi Taq DNA Polymerase is a mixture of Taq and Pfu DNA Polymerase. It is used to improve the reliability and yield of conventional primer extension reaction.
CombiTaq has two following advantages:
A, CombiTaq is twice the fidelity of Taq.
B, CombiTaq increase the efficiency of polymerization reaction. The fragments longer than 10kb can be amplified with Combi Taq.

The products of PCR contain a dA in the 3’end. All reagents, including Combi Taq, should be mixed well before use and use the mixture immediately after mixing. DNA synthesis is performed in 50uL of mixture containing 2.5U Combi Taq and 10ng-500ng template DNA, 100-300uM dNTP, 5uL of 10 x reaction buffer, 1.5-3.0mM Mg²⁺, 0.3-1uM primers.

Store at -20ºC. Stable for 12 months in constant freezer temperature.

Protein Experiment, Staining Reagent

C₄₇H₅₂N₃NaO₇S₂
M.W.: 858.05

Commonly used stain for the detection of protein bands following electrophoresis.
For staining with dye-suspension in TCA solution..

Store at Room Temp. Protect from moisture.

Protein Experiment, Staining Reagent

C₄₅H₄₈N₃NaO₇S₂
M.W.: 830.00

Tablets with 25mg Dye each
Tablet mass 100(+-5)mg average, 6mm dia, 2.5mm thick
For Staining: 4 Tablets in 250mL Methanol/Water/Acetic Acid 4/5/1

Store at Room Temp.