Molecular Biology Buffer

3-(CyclohexylAmino)-1-PropaneSulfonic Acid
C₉H₁₉NO₃S
M.W.: 221.32
Assay (from N): 99.13%
pKa (25℃): 10.26
Loss on Drying: 0.89%
A₂₅₀ (0.5M in water): 0.0135
A₂₈₀ (0.5M in water): 0.0129

CAPS is an alkaline buffer (pH 10-11, concentration 10-50mM, pH adjustment by NaOH) for protein transfer after SDS polyacrylamide gel electrophoresis on nitrocellulose filters.

Store at Room Temp.

DNA/RNA Experiment, For Density Gradient Centrifugation

CsCl
MW:168.36
99.9% Min.
A₂₆₀ (50% w/w H₂O): 0.023
A₂₈₀ (50% w/w H₂O): 0.018

For density gradient centrifugation, No enzymes, Low heavy metals.

Store at Room Temp.

DNA/RNA Experiment, For Density Gradient Centrifugation

CsCl
MW:168.36
99.999% Min.
A₂₆₀ (50% w/w H₂O): 0.009
A₂₈₀ (50% w/w H₂O): 0.008

Highest optical quality for density gradient centrifugation, No enzymes, Low heavy metals.

Store at Room Temp.

Molecular Biology Buffer, A Nondenaturing Zwitterionic Detergent for Membrane Biochemistry

3-[(3-Cholamidopropyl)Dimethylamino]-1-Propanesulfonate
C₃₂H₅₈N₂O₇S
M.W.: 614.88
Assay (from N): 99.94%
TLC: one spot
Loss on Drying: 0.96%
Solubility (10% in water, 25℃): Clear and colorless

CHAPS allows the isolation of many proteins in their native state, without loosing much of its activity. Protein-protein interactions can be interrupted more efficiently as with Triton X-100 or sodium cholate.

CHAPS interferes with the Lowry protein assay.

Working Conc.: 1-13mM

Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-6370
A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis.

Store at 4℃

Molecular Biology Buffer

2-(CycloHexylamino)-Ethane Sulfonic Acid
C₈H₁₇NO₃S
M.W.: 207.29
Assay (Titration): 99.62%
A₂₆₀ (0.1M in water): 0.0078
A₂₈₀ (0.1M in water): 0.0061

CHES interferes with the Lowry assay.

Ellis, K.J. & Morrison, J.F. (1982) Methods Enzymol. 87, 405-426
Buffers of constant ionic strength for studying pH-dependent processes.

Store at Room Temp.

Protein Experiment, Staining Reagent

C₄₇H₅₂N₃NaO₇S₂
M.W.: 858.05

For staining with dye-suspension in TCA solution.
Each tablet contains 50 mg dye and 50 mg salts. Prices refer to product weights.

Store at Room Temp.

Protein Experiment, Staining Reagent

C₄₅H₄₈N₃NaO₇S₂
M.W.: 830.00

For staining with dye-suspension in ethanol-acetic acid solution.
Each tablet contains 50 mg dye and 50 mg salts. Prices refer to product weights

Store at Room Temp.