RNA Experiment, For RNA Isolation

Ready-to-use reagent for the isolation of total RNA from cells and tissues.

Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156-159
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Store at 4℃

Toxic

Yeast Selection Reagent

C₅H₃FN₂O₄
M.W.: 174.09
Typical Purity (HPLC): >98%
Orotic Acid (HPLC): <2%
5-Fluorouracil: None detected
Melting point: 278℃
Solubility: 50mg/mL (4M NH₂OH)

5-Fluoroorotic acid is a pyrimidine analogue and used for the selection of Saccharomyces cerevisiae with mutations in the URA3-locus (ura3^--cells) and Schizosaccharomyces pombe. Wilde type strains of yeast or mutants with a plasmid carrying the URA3-sequence cannot grow in the presence of 5-FOA. Mutations in the URA3-gene result in the loss of activity of the Orotidin-5'-phosphate decarboxylase.

Working Conc.: 1mg/mL - 500ug/mL (500ug/mL will get higher background)

Normally a minimal medium for 5-FOA-selection is used, since in rich medium (YPD) different URA3⁺-strains may grow in the presence of 5-FOA.

Store at -20℃

Harmful